International Science Community Association Research Journal of Chemical Sciences 2231 - 606X 7 7 2017 07 18 In vitro anti-collagenase activity of Sri Lankan low grown orthodox Orange Pekoe grade black tea (Camellia sinensis L.) 11 15 EN Seleem H.S. 1 Department of Chemistry, Faculty of Education, Ain Shams University, Roxy, Cairo, Egypt El-Inany G.A. 2 Department of Chemistry, Faculty of Education, Ain Shams University, Roxy, Cairo, Egypt El-Shafiy H. 3 Department of Chemistry, Faculty of Education, Ain Shams University, Roxy, Cairo, Egypt Mousa M. 4 Department of Chemistry, Faculty of Education, Ain Shams University, Roxy, Cairo, Egypt Madyan A. 5 Department of Chemistry, Faculty of Education, Ain Shams University, Roxy, Cairo, Egypt El-Shetary B.A. 6 Department of Chemistry, Faculty of Education, Ain Shams University, Roxy, Cairo, Egypt Axel Lontsi Tiwa 1 Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O. Box 6163, Yaoundé, Cameroon and Department of Organic Chemistry, University of Yaoundé I, P.O Box 812, Yaoundé, Cameroon Alembert Tiabou Tchinda 2 Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O. Box 6163, Yaoundé, Cameroon Ratnasooriya W.D. 1 Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Ratmalana, Sri Lanka and Department of Zoology, University of Colombo, Colombo-03, Sri Lanka Abeysekera W.P.K.M. 2 Herbal Technology Section, Industrial Technology Institute (ITI), 363, Bauddhaloka Mawatha, Colombo 07, Sri Lanka Premakumara G.A.S. 3 Herbal Technology Section, Industrial Technology Institute (ITI), 363, Bauddhaloka Mawatha, Colombo 07, Sri Lanka Ratnasooriya C.D.T. 4 Faculty of Medicine, University of Colombo, Colombo-08, Sri Lanka Ratnasooriya S.G. 5 Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka > Research Paper 2017 5 17 2017 07 18 Anti-collagenase activity of tea brew of Sri Lankan low grown orthodox Orange Pekoe grade black tea (Camellia sinensis L.) was evaluated using five concentrations (25, 50, 75, 100 and 200 µg/ml) of black tea brew (BTB) made according to ISO specifications. Anti-collagenase activity of BTB and tea catechin, epigallocatechingallate (EGCG) were ascertained in vitro using collagenase enzyme from Clostridium histolyticum and a synthetic substrate (FALGPA) using spectroscopy. BTB induced marked and significant (P < 0.05) anti-collagenase activity (IC50 = 80.04 ħ 2.34 µg/ml). This effect was dose dependent. Moreover, anti-collagenase activity of BTB was superior to EGCG (IC50 = 112.12 ħ 0.93 µg/ml), a well known anti-collagenase phytoconstituent of green tea. The results convincingly show that Sri Lankan low grown orthodox Orange Pekoe grade black tea possesses remarkable anti-collagenase activity in vitro and display its promise to be developed as a potent anti-aging skin nutraceutical. Copyright@ International Science Community Association