International Science Community Association Research Journal of Chemical Sciences 2231 - 606X 2 7 2012 July 18 Potential Recovery of Protein from Shrimp Waste in Aqueous two Phase System 47 52 EN Kumar Harish 1 Material Science Laboratory, Department of Chemistry, Ch. Devi Lal University, Sirsa, Haryana, INDIA Sunita 2 Department of Chemistry, Singhania University, Rajasthan, INDIA Diwya 1 Acharya institute of Technology, Soldevanahalli, Hesarghatta Road, Bangalore-560090, Karnataka, INDIA Iyengar Pushpa 2 Department of Chemistry, Jyoti Nivas College, Koramangala Industrial Layout, Bangalore - 560095, Karnataka, INDIA Ramachandrappa R. 3 Bobade S.N. 1 Indian Biodiesel Corporation, Baramati, above Sh. Malojiraje Co-op. Bank, Tal- Baramati, Dist- Pune, MS, INDIA Shardabai Pawar Mahila College, Khyade V.B. 2 Shardanagar, Tel. Baramati, Dist – Pune, MS, INDIA Mihayo I.Z. 1 Dept. of Physical Sci, Faculty of Science, Sokoine University of Agriculture, Chuo Kikuu, Morogoro, TANZANIA Mkoma S.L. 2 Kannan C. 1 Department of Chemistry, Manonmaniam Sundaranar University, Tirunelveli-627012, Tamilnadu, INDIA Devi M.R. 2 Muthuraja K. 3 Esaivani K. 4 SudalaiVadivoo V. 5 Mishra Subhra 1 Department of Chemistry, Alipurduar College, North Bengal University, Pin-736122, West Bengal, INDIA Sunakar Panda 1 P.G.Dept of Chemistry,Berhampur University, Ganjam-760007,Odisha, INDIA Chandra PanigrahiJagadish 2 Pulp and Paper Research Institute, Jaykaypur, Rayagada-765017, Odisha, INDIA Tripathy UpendraPrasad 3 Ramyadevi D. 1 Department of Chemical Engineering, National Institute of Technology, Tiruchirappalli- 620 015, INDIA Subathira A. 2 Saravanan S. 3 > Research Paper 2012 4 7 2012 July 18 Shrimp waste is an important source of bioactive molecules and it undergoes rapid disintegration which leading to environmental pollution. It is necessary to preserve the material adopting the environmentally safe techniques, prior to recovery of bioactive components such as proteins and carotenoids. Aqueous two-phase system (ATPS) partitioning has been used to recover and concentrate proteins from Shrimp Waste and offers many advantages along with biomass removal. Hence, a feasible protocol for the recovery of protein from Shrimp Waste has been established. The best conditions of partitioning were achieved using ATPS composed of PEG 4000, ammonium citrate salt, pH8, 1M addition of sodium chloride, and 36.28 Tie line length. The maximum percentage yield of protein extracted from shrimp waste was found to be 74.50%. Copyright@ International Science Community Association