International Science Community Association Research Journal of Chemical Sciences 16 1 2026 02 18 Assay Method Development and Validation of Biclotymolby High Performance Liquid Chromatography (HPLC) 52 62 EN Gupta Swaroopa Rani N. 1 Department of Chemistry, Brijlal Biyani Science College, Amravati, Maharashtra India Thakor Subhash B. 1 Shri PHG Municipal Arts and Science College, Gujarat University, Kalol – 382721, Gujarat, India Nyong Aniedi E. 1 Akwa Ibom State University, Department of Chemistry, Physical and Materials Chemistry Unit, P.M.B 1167, Post Code 532111, Ikot Akpaden, Akwa Ibom State, Nigeria Obadimu Clement O. 2 Akwa Ibom State University, Department of Chemistry, Physical and Materials Chemistry Unit, P.M.B 1167, Post Code 532111, Ikot Akpaden, Akwa Ibom State, Nigeria Alexander Joseph 3 Akwa Ibom State University, Department of Chemistry, Physical and Materials Chemistry Unit, P.M.B 1167, Post Code 532111, Ikot Akpaden, Akwa Ibom State, Nigeria George Wisdom 4 Akwa Ibom State University, Department of Chemistry, Physical and Materials Chemistry Unit, P.M.B 1167, Post Code 532111, Ikot Akpaden, Akwa Ibom State, Nigeria Udo Godwin J. 5 Akwa Ibom State University, Department of Chemistry, Physical and Materials Chemistry Unit, P.M.B 1167, Post Code 532111, Ikot Akpaden, Akwa Ibom State, Nigeria Awaka-Ama Joachim J. 6 Akwa Ibom State University, Department of Chemistry, Physical and Materials Chemistry Unit, P.M.B 1167, Post Code 532111, Ikot Akpaden, Akwa Ibom State, Nigeria Girmay Hillary 1 Department of Plant Science, College of Agriculture, Hawassa University, Ethiopia Adane Legesse 2 Department of Chemistry, College of Computational and Natural Science, Hawassa University Tesema Meseret 3 Department of Plant Science, College of Agriculture, Hawassa University, Ethiopia Worku Yishak 4 Department of Food Science, College of Agriculture, Hawassa University, Ethiopia KPOTIN Assongba Gaston 1 Laboratory of Physical Chemistry-Materials and Molecular Modelling (LPC3M), Unit of Theoretical Chemistry and Molecular Modelling (UTC2M), University of Abomey-Calavi (UAC)/Benin AMOUSSOU Maounou Boris 2 Laboratory of Physical Chemistry-Materials and Molecular Modelling (LPC3M), Unit of Theoretical Chemistry and Molecular Modelling (UTC2M), University of Abomey-Calavi (UAC)/Benin KANKINOU Sèlonou Gautier 3 Laboratory of Physical Chemistry-Materials and Molecular Modelling (LPC3M), Unit of Theoretical Chemistry and Molecular Modelling (UTC2M), University of Abomey-Calavi (UAC)/Benin ATOHOUN Guy Sylvain Yacole 4 Laboratory of Physical Chemistry-Materials and Molecular Modelling (LPC3M), Unit of Theoretical Chemistry and Molecular Modelling (UTC2M), University of Abomey-Calavi (UAC)/Benin Pandey Sunil Kumar 1 Department of Applied Science, Dr. K.N. Modi University, Newai, Rajasthan, India Sheoran Manisha 2 Department of Applied Science, Dr. K.N. Modi University, Newai, Rajasthan, India Sharma R.N. 3 Department of chemistry, S.H.S Government P.G College, Dhanapur, Chandauli, India > 2025 11 28 2026 02 18 Biclotymol is a phenolic antiseptic API molecule which generally use for the treatment of mouth and throat infection. It is antibacterial, anti-inflammatory and analgesic. Due to phenolic antiseptic it kills bacteria, Biclotymol have fast acting and long lasting molecule. The Method Validation is applicable for demonstration of method accuracy, method precision, linearity, repeatability, reproducibility, limit of detection(LOD) and limit of quantitation(LOQ) for analysis of Biclotymol for assay. High Performance Liquid Chromatography(HPLC) equipped with VWD detector and stainless steel column 25 cm×4.6 mm, C18 (5µm). From the analytical method validations report, it is concluded that the Assay of Biclotymol is (96.0%- 101.0%). Copyright@ International Science Community Association