International Science Community Association Research Journal of Chemical Sciences 16 1 2026 02 18 Estimation of free fatty acids, iodine value and saponification value of sea oil and its FTIR study 1 6 EN Gupta Swaroopa Rani N. 1 Department of Chemistry, Brijlal Biyani Science College, Amravati, Maharashtra India > 2024 11 5 2026 02 18 Sea buckthorn oil is red-orange oil derived from sea buckthorn plants. Oil content in seeds of sea buckthorn is on average 7–11 % while oil content of the fruit pulp is around 1.5–3%. Seed oil is characterized by high contents of polyunsaturated fatty acids while pulp oil contains monounsaturated fatty acids and carotenoids. Both oils also contain dense amounts of tocopherols, tocotrienols and plant sterols. Present Paper deals with Estimation of Free Fatty acids, Iodine value and Saponification value of Sea Buckthorn Oil and FTIR study of Sea Buckthorn Oil. Fat or oil contains small quantity of free fatty acids. On storing, the free fatty acid contents of the fat or oil increases. The free fatty acid contents are determined by direct titration of fat or oil with standard KOH solution. The acid value is defined as the numbers of milligrams of KOH required to neutralize the free fatty acid present in 1 g of the fat or oil. The iodine value of oil is the number of grams of iodine taken up by 100 g of the oil. It is determined by reacting a known volume of excess solution of iodine monochloride in acetic acid (Wij's solution) with oil and then back titrating unreacted iodine with Sodium thiosulphate solution. Saponification value of an oil or fat is defined as the number of milligrams of Potassium Hydroxide required to hydrolyse (saponify) one gram of oil completely. A known amount of oil is refluxed with excess amount of standard alcoholic potash solution and the unused alkali is titrated against standard acid solution using phenolphthalein as an indicator. Copyright@ International Science Community Association