International Science Community Association Research Journal of Chemical Sciences 15 1 2025 02 18 Green Nanotechnology synthesized silver Nanoparticles: characterization and testing its antibacterial activity 8 15 EN Zaware Sandeep 1 Synergen Bio Private Limited, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India Lahoti Neel 2 Synergen Bio Private Limited, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India Shinde Hrushikesh 3 Synergen Bio Private Limited, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India Bhatia Swati 1 Sage University, Indore, India Upadhyay Siya 2 Sage University, Indore, India > Research Paper 2024 5 16 2025 02 18 Developing eco-friendly strategies “for metallic nanoparticle synthesis using natural extracts has gained significant traction. This study explores the potential of Carica papaya leaf extract for the biogenic synthesis of metallic nanoparticles and evaluates their subsequent antimicrobial activity. A reduction reaction under ambient conditions, employing leaf extract, facilitated the nanoparticle formation from metal salts. Characterisation techniques encompassing UV-Vis spectroscopy, FTIR, XRD, and TEM corroborated the successful synthesis of well-dispersed, spherical nanoparticles. The presence of biomolecules within the extract was identified as crucial for both nanoparticle reduction and stabilisation, as evidenced by FTIR analysis. XRD patterns confirmed the crystalline nature of the nanoparticles, with distinct peaks corresponding to specific crystal planes. This antimicrobial efficacy is attributed to the synergistic action between the metallic nanoparticles themselves and the inherent bioactive compounds present within the Carica papaya leaf extract.” In conclusion, this work presents a facile and environmentally benign approach for nanoparticle synthesis utilising Carica papaya leaf extract. The synthesised nanoparticles demonstrate promising antimicrobial properties, highlighting the potential of natural extracts for the development of novel biocompatible nanomaterials with diverse applications in the biomedical and environmental fields. Further research is warranted to elucidate the precise mechanisms underlying their antimicrobial action and explore their potential therapeutic applications. Copyright@ International Science Community Association