International Science Community Association Research Journal of Chemical Sciences 2231-606X 12 3 2022 10 18 A study of binding of DNA extracted from onion with Ruthunium(II) Polypyridyl Complexes 20 24 EN S. Suresh Kumar 1 Department of Biotechnology, P.S.R. Engineering College, Sivakasi-626140, Tamilnadu, India Srinivasan Venkatesh 2 Department of Biotechnology, P.S.R. Engineering College, Sivakasi-626140, Tamilnadu, India R. Subashini 3 Department of Biotechnology, P.S.R. Engineering College, Sivakasi-626140, Tamilnadu, India Karuppasamy Venkadeswaran 4 Department of Biotechnology, P.S.R. Engineering College, Sivakasi-626140, Tamilnadu, India S. Nithya 5 Department of Biotechnology, P.S.R. Engineering College, Sivakasi-626140, Tamilnadu, India B. Padmapriya 6 Department of Biotechnology, P.S.R. Engineering College, Sivakasi-626140, Tamilnadu, India R. Sumitha 7 Department of Biotechnology, P.S.R. Engineering College, Sivakasi-626140, Tamilnadu, India Emmanuel Okhimamhe Akhalumhe 1 Gramen Petroserve Nigeria Limited, Port Harcourt, Nigeria Giegbefumwen Peter Uyi 2 Department of Petroleum Engineering, University of Benin, Benin City, Nigeria Mitra Sanchita 1 Department of Biotechnology, Manav Rachna International Institute of Research and Studies, Faridabad, Haryana India, and Department of Chemistry, Gargi, College New Delhi, India Tomar Pushpa C. 2 Department of Biotechnology, Manav Rachna International Institute of Research and Studies, Faridabad, Haryana India, and Department of Chemistry, Gargi, College New Delhi, India M. Abisha 1 Department of Chemistry and Research Centre, Scott Christian College (Autonomous) Nagercoil, Tamilnadu, India T. Sumitha Celin 2 Department of Chemistry and Research Centre, Scott Christian College (Autonomous) Nagercoil, Tamilnadu, India G. Allen Gnana Raj 3 Department of Chemistry and Research Centre, Scott Christian College (Autonomous) Nagercoil, Tamilnadu, India > Research Paper 2022 3 16 2022 10 18 In an aqueous solution, the binding of the Ru(II) polypyridyl complex [RuL3]2+ (where L=bpy, dmbpy) with onion DNA has been investigated using absorption and emission spectrum methods. The MLCT absorption maxima for both compounds are in the 445–460nm region. Using data on absorption intensity and emission investigations, the binding constant (Kb) for these processes is calculated from the Benesi-Hildebrand equation. The luminophore and DNA molecule interact at the ground state in a hydrophobic way. These complexes bind to DNA through intercalative mode. The Kb value depends on the nature of the DDNA and also on the ligand used. Copyright@ International Science Community Association