International Science Community Association Research Journal of Engineering Sciences 2278-9472 13 3 2024 09 26 Exploring the Potential of Andrographis Paniculata Extract as a Natural Antimicrobial Agent on Cotton Fabric: Insights from FTIR, UV Spectroscopy, and Phytochemical Analysis 1 10 EN Prajapati Parul 1 Department of Textile Chemistry, Faculty of Technology and Engineering, Kalabhavan, The Maharaja Sayajirao University of Baroda, Vadodara – 390 001, Gujarat, India Patel Bharat 2 Department of Textile Chemistry, Faculty of Technology and Engineering, Kalabhavan, The Maharaja Sayajirao University of Baroda, Vadodara – 390 001, Gujarat, India > Research Paper 2024 2 19 2024 09 26 Consumers’ attitude toward hygiene and an active lifestyle have created a rapidly increasing market for antimicrobial textiles, which in turn has stimulated intensive research and development. Being in contact with the skin and a barrier between outer space and the body, it can accumulate and inculcate various bacteria and viruses. A large number of synthetic antimicrobial agents are available but they come with the disadvantages of increasing pollution load, drug resistance factors, and many more. This study focuses on the evaluation of the antimicrobial activity of cotton fabric treated with the herbal bioactive agent Andrographis paniculata. Phytochemical Analysis, FTIR, and UV spectroscopy results showed the presence of active bioactive compounds responsible for antimicrobial activity. The bioactive agent from Andrographis Paniculata was extracted with the Soxhlet extraction technique and applied to cotton fabric. The quantitative antimicrobial activity showed 99.90% bacterial growth reduction against Gram-positive and Gram-negative bacteria. Copyright@ International Science Community Association