Molecular barcode analysis and phylogeny of parasitic helminthes: A laboratory manual
- 1Dept. of Biotechnology, M.M. Engineering College, Maharishi Markandeshwar Deemed University Mullana, Ambala-133 207, Haryana, India
Res. J. Recent Sci., Volume 7, Issue (3), Pages 22-27, March,2 (2018)
As all eukaryotic organisms, helminthes also have Deoxyribonucleic acid (DNA) as hereditary genetic material. The DNA mostly located in the nucleus of cell (nuclear DNA), but a little quantity of DNA can also be found in mitochondria (mitochondrial DNA or mtDNA). DNA of organisms is a unique feature with encoded information which describes all phenotypic and physiological characteristics of bearing animals like barcode of a physical object. Paul Hebert, researcher at the University of Guelph in Ontario, Canada, proposed “DNA barcoding” as a contrivance to identify biological species in 2003. Until now, organisms were identified using morphological characters like shape, size and color of body parts. There was a skilled researcher could make routine observations and microscopically identify specimens using morphological “keys”, but in several cases a learned and eminent taxonomist was considered necessary. When a specimen was mechanically or physically broken or if in an immature stage of development then even experts might not be able to made proper identifications. The validation of taxa was performed to remove ambiguity in identification of cestodes and nematodes by phylogenetic analysis, which was earlier studied by using morphotaxometric analyses. DNA barcoding resolves these ambiguities of morphological identifications because even sub-proficient worker can acquire barcodes from a little amount of tissue. Author wish to explore by the present work about ambiguities in traditionally identified organisms, which can be evaluated and validated by barcoding, phylogeny and molecular heterogeneity, so that individuals may place at appropriate taxonomic status.
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