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Simultaneous validation of amoxicillin trihydrate and carbocisteine in pharmaceutical dosage by reverse phase high performance liquid chromatography

Author Affiliations

  • 1Central Research Laboratory, Chemistry, D.G. Ruparel College, Dept. of Chemistry, Matunga, Mumbai-400 016, India
  • 2Central Research Laboratory, Chemistry, D.G. Ruparel College, Dept. of Chemistry, Matunga, Mumbai-400 016, India

Res. J. Recent Sci., Volume 7, Issue (3), Pages 8-13, March,2 (2018)

Abstract

For validation of amoxicillin trihydrate and carbocisteine from the formulation i.e. capsules, a RP-HPLC method is developed. The amoxicillin trihydrate and carbocisteine were resolved on a BDS hypersil C18 (150 x 4.6 mm i.d.) 5 particle size. The water and methanol in the ratio of 80: 20 % (v/v) constituted as mobile phase with detection wavelength as 220 nm. The column BDS hypersil C18 was found to be most suitable for chromatographic separation. The parameters for robustness were validated. The linear ranges amoxicillin trihydrate as 125 - 375 and carbocisteine at 75 - 225 &

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