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Isolation, identification and biotyping of brucella abortus in cattle and camels in Northern State, Sudan

Author Affiliations

  • 1Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University of Dongola, Sudan

Res. J. Recent Sci., Volume 6, Issue (3), Pages 32-34, March,2 (2017)

Abstract

Brucellosis is a thropo-zoonotic infection which can be considered as a great challenge to development of dairy production in developing countries. It is one of the most economically devastating disease, which causes heavy economical losses resulting both from direct effects on animals such as abortions, irregular breeding, sterility and decreased milk yield. From indirect effects on the animal industry associated with costs of veterinary attendance, replacement of animals and impediment to free animal movement and export. The objective of this study was to isolate and characterize Brucella species and determine the biovars associated with the disease. Seven Brucella a bortus biovar l was isolated and identified from cattel and camels in Northern State, Sudan. Tow isolates were isolated from bovine milk. Four from bovine vaginal swabs and one from a placental specimens from a camel. Placental fragment, vaginal swabs, milk and hygroma fluids were collected. Then cultured into Mueller Hinton agar medium. Isolates were identified as Brucella (spp) on the basis of morphology, staining of the organism, production of catalase, oxidase and urease. The colonies appeared after 4-5 days of incubation and were smooth and transparent. Suspected colonies were stained by Gram stain used Modified Zihl-Neelsen (MZN) methods. Gram- negative coccobacilli were found in smears from the colonies. All biochemical tests of the showed characteristics of brucella. The isolates were agglutinated with Brucella A but not with M Brucella antiserum and lyses by Wb, Tb, Bk and Fi phages. We concluded that the seven isolates were sensitive to the antibodies used for treatment of the disease and one isolate show in vitro resistance to Rifampicin.

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