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Determination of divalent Mercury in environmental samples using 1, 5-diphenyl-3-thiocarbazone: with modified, ultrasensitive, direct Spectrophotometric method

Author Affiliations

  • 1Department of Environmental Science and Research Centre, K.R.T. Arts, B.H. Commerce and A.M. Science College, Nashik, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India
  • 2Departments of Microbiology, K.R.T. Arts, B.H. Commerce and A.M. Science College, Nashik, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India
  • 3Department of Botany, K.R.T. Arts, B.H. Commerce and A.M. Science College, Nashik, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India

Res. J. Recent Sci., Volume 6, Issue (3), Pages 8-12, March,2 (2017)

Abstract

The present research investigated that, the analytical reagent,1,5-diphenyl-3 thiocarbazone dissolves in acetone in strongly acidic and 1,4 dioxane media which reacts with mercury (II) to produce orange-red coloured complex at pH 2 (0.2N sulphuric acid). The complex was showing maximum absorbance at 488 nm. Therefore, further analytical parameters were performed at 488nm. The reaction occurs within a minute and absorbance remains unchanged for 24 hrs. The analytical parameters like, effect of metal concentration, reagent concentration, acidity, interference by other metal ions, were investigated. The Lambert-Beer’s law followed within a range of 0.1-25 µgml-1of Hg(II). The stoichiometric ratio of a reagent with the reacting metal is 1:2 (Mercury:Dithiazone). The molar absorptivity was found to be 2.4×104 lmol-1cm-1 and that of specific absorptivity was 0.015µg of mercury (II)/cm2. The selected method is modified, ultrasensitive and is successfully applied for estimation of divalent mercury from polluted water samples.

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