Effect of extraction temperature and technique on phenolic compounds and antioxidant activity of Tamarindus indica seeds
- 1Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata – 700032, India
- 2Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata – 700032, India
Res. J. Recent Sci., Volume 6, Issue (2), Pages 10-15, February,2 (2017)
Natural antioxidants from tamarind seed pose preservative effect in food and disease protective benefits in biological system. Extraction being the initial and crucial most step of antioxidant recovery is influenced by many conditions. In this study the effects of technique and temperature during solvent extraction of phenolic antioxidants from tamarind seed have been studied. The extraction techniques compared have been shaking and magnetic stirring. A binary solvent system of 50 % ethanol has been used and extraction has been carried out at varied temperatures (ranging 25 - 60° C). Total Polyphenol Content (TPC) and Total Antioxidant Activity (TAA) determined at these conditions have indicated magnetic stirring as the most convenient and exhaustive technique. While the maximum TPC has been extractable at 60° C, the highest TAA has been recorded at the extraction temperature of 40 ° C. Results have indicated that extracting temperature and technique significantly (P<0.05) influenced the antioxidant property and total phenolic compound recovery from tamarind seed.
- Riley P.A. (1994)., Free radicals in biology: oxidative stress and the effects of ionizing radiation., International journal of radiation biology, 65(1), 27-33.
- Sies H. (1991)., Oxidative stress: from basic research to clinical application., The American journal of medicine, 91(3), S31-S38.
- Perumalla A.V.S. and Hettiarachchy N.S. (2011)., Green tea and grape seed extracts-Potential applications in food safety and quality., Food Research International, 44(4), 827-839.
- Sudjaroen Y., Haubner R., Würtele G., Hull W.E., Erben G., Spiegelhalder B., Changbumrung S., Bartsch H. and Owen R.W. (2005)., Isolation and structure elucidation of phenolic antioxidants from Tamarind (Tamarindus indica L.) seeds and pericarp., Food and Chemical Toxicology, 43(11), 1673-1682.
- El-Siddig K. (2006)., Tamarind Tamarindus indica L., Fruits for the future 1 revised edition, Southampton Centre for Underutilised Crops, England, Crops for the Future, 1,
- Rao A.S. and Kumar A.A. (2015)., Tamarind seed processing and by-products., Agricultural Engineering International: CIGR Journal, 17(2), 200-204.
- Moure A., Cruz J.M., Franco D., Domı́nguez J.M., Sineiro J., Domı́nguez H., Núñez M.J. and Parajó J.C. (2001)., Natural antioxidants from residual sources., Food chemistry, 72(2), 145-171.
- Hayouni E.A., Abedrabba M., Bouix M. and Hamdi M. (2007)., The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts., Food Chemistry, 105(3), 1126-1134.
- Lapornik B., Prošek M. and Wondra A.G. (2005)., Comparison of extracts prepared from plant by-products using different solvents and extraction time., Journal of food engineering, 71(2), 214-222.
- Spigno G., Tramelli L. and De Faveri D.M. (2007)., Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics., Journal of food engineering, 81(1), 200-208.
- Pinelo M., Rubilar M., Jerez M., Sineiro J. and Núñez M.J. (2005)., Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace., Journal of Agricultural and Food Chemistry, 53(6), 2111-2117.
- Musa K.H., Abdullah A., Jusoh K. and Subramaniam V. (2011)., Antioxidant activity of pink-flesh guava (Psidium guajava L.): effect of extraction techniques and solvents., Food Analytical Methods, 4(1), 100-107.
- Ismail A. and Tan S.H. (2002)., Antioxidant activity of selected commercial seaweeds., Malaysian Journal of Nutrition, 8(2), 167-177.
- Khiari Z., Makris D.P. and Kefalas P. (2009)., An investigation on the recovery of antioxidant phenolics from onion solid wastes employing water/ethanol-based solvent systems., Food and Bioprocess Technology, 2(4), 337-343.
- Akowuah G.A., Ismail Z., Norhayati I. and Sadikun A. (2005)., The effects of different extraction solvents of varying polarities on polyphenols of Orthosiphon stamineus and evaluation of the free radical-scavenging activity., Food chemistry, 93(2), 311-317.
- Sarkar A. and Ghosh U. (2016)., Classical Single Factor Optimisation of Parameters for Phenolic Antioxidant Extraction from Tamarind Seed (Tamarindus indica)., Plant Science Today, 3(3), 258-266.
- Malik C.P. and Singh M.B. (1980)., Plant enzymology and histo-enzymology., Kalyani Publishers, New Delhi, India, 434.
- Benzie I.F.F. and Strain J.J. (1996)., The ferric reducing ability of plasma (FRAP) as a measure of \\\"antioxidant power\\\": The FRAP assay., Anal Biochem, 239(1), 70–76.
- Suh Y.K. and Kang S. (2010)., A review on mixing in microfluidics., Micromachines, 1(3), 82-111.
- Clarke Gail Kim (2013)., Bioprocess Engineering: An introductory Engineering and Life Science Approach,, Woofhead Publishing LTD., USA, 227- 230.
- Sultana B., Anwar F. and Ashraf M. (2009)., Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts., Molecules, 14(6), 2167-2180.
- Yen G.C. and Chang Y.C. (2003)., Production of antioxidant from Aspergillus candidus broth filtrate by fermentor., Process Biochemistry, 38(10), 1425-1430.
- Al-Farsi M.A. and Lee C.Y. (2008)., Optimisation of phenolics and dietary fibre extraction from date seeds., Food Chem, 108(3), 977-985.
- Mandal S., Mitra A. and Mallick N. (2009)., Time course study on accumulation of cell wall-bound phenolics and activities of defense enzymes in tomato roots in relation to Fusarium wilt., World journal of Microbiology and Biotechnology, 25(5), 795-802.
- Chan E.W.C., Lim Y.Y., Wong S.K., Lim K.K., Tan S.P., Lianto F.S. and Yong M.Y. (2009)., Effects of different drying methods on the antioxidant properties of leaves and tea of ginger species., Food Chem., 113(1): 166-172.
- Dewanto V., Wu X., Adom K.K. and Liu R.H. (2002)., Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity., Journal of agricultural and food chemistry, 50(10), 3010-3014.
- Haynes William M. (2014)., CRC Handbook of Chemistry and Physics,, 95nd ed., CRC Press, USA.