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Reverse Phase High Performance Liquid Chromatography for Simultaneous Determination of Aceclofenac and Thiocolchicoside in Bulk and Pharmaceutical Dosage form

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Author Affiliations

  • 1Central research laboratory, D.G. Ruparel College, Matunga, Mumbai, India
  • 2Central research laboratory, D.G. Ruparel College, Matunga, Mumbai, India

Res. J. Recent Sci., Volume 5, Issue (ISC-2015), Pages 85-89, -----Select----,2 (2016)

Abstract

A simple, rapid and accurate high performance liquid chromatography method is described for simultaneous determination of aceclofenac and thiocolchicoside from combined dosage form i.e. tablets. The separation of drug was achieved on Polaris C18 (150 x 4.6 mm i.d.) with 5 µ particle size, column showed most favorable chromatographic pattern over the other columns. The mobile phase consisted of a mixture of buffer and acetonitrile (55:45 % (v/v)). The buffer was 0.01 M ammonium acetate solution adjusted the pH 3.4 with ortho-phosphoric acid. The detection was carried out at wavelength 270 nm. The mixture of buffer of pH 3.4 and acetonitrile (55:45% v/v) was used as a diluent. The method was validated for system suitability, linearity, accuracy, precision, robustness, stability of sample solution. The method has been successfully used to analyze aceclofenac and thiocolchicoside from combined dosage form i.e. tablets.

References

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