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Docking Studies of Heme Ligand onto the predicted 3D Structure of Fatty Acid Desaturase 2 from Rat

Author Affiliations

  • 1University of Rwanda, College of Science and Technology, Department of Biology, Avenue de l’Armée, Po. Box 3900, Kigali-Rwanda
  • 2University of Rwanda, College of Science and Technology, Department of Biology, Avenue de l’Armée, Po. Box 3900, Kigali-Rwanda
  • 3University of Rwanda, College of Science and Technology, Department of Biology, Avenue de l’Armée, Po. Box 3900, Kigali-Rwanda
  • 4University of Rwanda, College of Science and Technology, Department of Biology, Avenue de l’Armée, Po. Box 3900, Kigali-Rwanda

Res. J. Recent Sci., Volume 5, Issue (9), Pages 30-37, September,2 (2016)

Abstract

Fatty acid desaturase 2 is a membrane bound enzyme of fatty acid desaturase family. It is encoded by a gene Fads2 located on the chromosome 1 of the rat genome. Like other membrane bound protein it has important physiological and industrial importance. Most importantly, the synthesis of polyunsaturated fatty acids requires the presence of fatty acid desaturase 2. However, the lack of its three-dimensional structure hinders the understanding of its biological function at molecular level. Sequence analysis was done using sequence alignment and phylogenetic which shows the evolutionary inferences between the sequence under study and its homologous proteins retrieved from PDB databank. To investigate in its functions, docking studies of heme ligand onto the predicted 3D structure of Fatty acid desaturase 2 from rat was conducted using computational methods. The ligand got docked onto the binding sites of the predicted model with more or less similar interacting residues when compare to interacting residues between heme and the experimentally determined structure of the template protein used while building the 3D model of fatty acid desaturase 2 through homology modeling technique.

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