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In silico analysis and comparison of phytase gene from Aspergillus niger

Author Affiliations

  • 1Department of Biotechnology, Faculty of Biological Sciences, University of South Asia, Lahore, Pakistan
  • 2Department of Biotechnology, Faculty of Biological Sciences, University of South Asia, Lahore, Pakistan
  • 3Department of Biotechnology, Faculty of Biological Sciences, University of South Asia, Lahore, Pakistan

Res. J. Recent Sci., Volume 5, Issue (4), Pages 46-50, April,2 (2016)

Abstract

Phytase gene releases extracellular phytase which degrades phytate to release phosphorus. In plants, phosphorus is primarily stored as the phytate..Expressing phytase gene to upgrade the production of phosphors is common these days. The most commonly used source of phytase gene is Aspergillus niger. To properly use phytase gene for transgenic purposes, in silico analysis is crucial to know important aspects and parameter of gene. In this study, in silico analysis and comparison of Aspergillus niger phytase gene was done using different bioinformatics approaches to identify the phytase gene.

References

  1. Mullaney E.J., Daly C.B. and Ullah A.H. (2000)., Advances in phytase research., Adv. Appl. Microbiol., 47, 157–199.
  2. Lei X-G., Porres J.M., Mullaney E.J. and Brinch-Pedersen H. (2007)., Phytase: source, structure and application., Ind Enz., 505–529.
  3. Brinch-Pedersen H., Sψrensen L.D. and Holm P.B. (2002)., Engineering crop plants: getting a handle on phosphate., Trends Plant Sci.,7, 118–125.
  4. Brinch-Pedersen H., Hatzack F., Stφger E., Arcalis E. and Pontopidan K. (2006)., Heat-stable phytases in transgenic wheat (Triticumaestivum L.): deposition pattern, thermostability, and phytate hydrolysis., J Agric Food Chem., 54, 4624–4632.
  5. Brinch Pedersen H., Olesen A., Rasmussen S.K. and Holm P.B. (2000)., Generation of transgenic wheat (Triticumaestivum L.) for constitutive accumulation of an Aspergillus phytase., Mol Breeding., 6, 195–206.
  6. Chiera J.M., Finer J.J. and Grabau E.A. (2004)., Ectopic expression of a soybean phytase in developing seeds of Glycine max to improve phosphorus availability., Plant Mol Biol.,56, 895–904.
  7. Bilyeu K.D., Zeng P., Coello P., Zhang Z.J. and Krishnan H.B. (2008)., Quantitative conversion of phytate to inorganic phosphorus in soybean seeds expressing a bacterial phytase., Plant Physiol.,146, 468–477.
  8. Ponstein A.S., Bade J.B., Verwoerd T.C., Molendijk L. and Storms J. (2002)., Stable expression of phytase (phyA) in canola (Brassica napus) seeds: towards a commercial product., Mol breeding, 10, 31–44.
  9. Peng R.H., Yao Q.H., Xiong A.S., Cheng Z.M. and Li. Y. (2005)., Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola., Plant Cell Rep., 25, 124–132.
  10. Chen R.M., Xue G.X., Chen P., Yao B. and Yang W.Z. (2008)., Transgenic maize plants expressing a fungal phytase gene., Transgenic Res., 17, 633–643.
  11. Haros M., Bielecka M. and Sanz Y. (2005)., Phytase activity as a novel metabolic feature in Bifidobacterium., FEMS Microbiol. Lett., 247, 231-239.
  12. Li M., Osak M., Madhusudana Rao I. and Tadano T. (1997)., Secretion of phytase from the roots of several plant species under phosphorus-deficient conditions., Plant Soil., 195, 161–169.
  13. Hayes J., Simpson R. and Richardson A. (2000)., The growth and phosphorus utilization of plants in sterile media when supplied with inositol hexaphosphate, glucose 1-phosphate or inorganic phosphate., Plant Soil., 220, 165–174.
  14. Richardson A.E., Hadobas P.A. and Hayes J.E. (2000)., Acid phosphomonoesterase and phytase activities of wheat (Triticumaestivum L.) roots and utilization of organic phosphorus substrates by seedlings grown in sterile culture., Plant Cell Environ., 23, 397–405.
  15. Idriss E.E., Makarewicz O., Farouk A., Rosner K. and Greiner R. (2002)., Extracellular phytase activity of Bacillus amyloliquefaciens FZB45 contributes to its plant-growth-promoting effect., Microbiol., 148, 2097–2109.
  16. Unno Y., Okubo K., Wasaki J., Shinano T. and Osaki M. (2005)., Plant growth promotion abilities and microscale bacterial dynamics in the rhizosphere of Lupin analysed by phytate utilization ability., Environ Microbiol.,7, 396–404.
  17. Richardson A.E., Hadobas P.A. and Hayes J.E. (2001)., Extracellular secretion of Aspergillus phytase from Arabidopsis roots enables plants to obtain phosphorus from phytate., Plant J., 25, 641–649.
  18. Mudge S.R., Smith F.W. and Richardson A.E. (2003)., Root-specific and phosphate-regulated expression of phytase under the control of a phosphate transporter promoter enables Arabidopsis to grow on phytate as a sole P source., Plant Sci., 165, 871–878.
  19. Aspergillus niger strain BCC18081 PhyA (phyA) mRNA, complete cds (2015)., GenBank: EU786167.1., http://www.ncbi.nlm.nih.gov/nuccore/EU7861672www.ncbi.nlm.nih.gov (2015).
  20. www.ncbi.nlm.nih.gov (2015)., https://www.ncbi.nlm.nih. gov/Structure/seqr/, /view/190589932/tree
  21. Protein: B3VPB3_ASPNG (B3VPB3) (2015)., http://pfam.xfam.org/protein/B3VPB3
  22. Leung M. (2004)., Persistence of β-propeller phytase in soil and its implication in phosphorus mobilization, The HKU Scholar Hub, University of Hong Kong., Zoology Thesis.
  23. Morya V.K., Yadav S., Kim E.K. and Yadav D. (2012)., In silico characterization of alkaline proteases from different species of Aspergillus., Applied Biochem and Biotechnol., 166, 243–257.