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Study on Antimicrobial Resistance of Escherichia Coli o157:H7/NM isolated from Raw Bovine, Camel, Water Buffalo, Caprine and Ovine milk

Author Affiliations

  • 1School of Veterinary Medicine, Shiraz University, Shiraz, IRAN
  • 2 College of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, IRAN

Res. J. Recent Sci., Volume 4, Issue (4), Pages 20-22, April,2 (2015)

Abstract

Escherichia coli O157:H7 has appeared as a food-producing pathogen in public health. Previously the various patterns of E.coli‘s transition in bovine cattle have been mentioned such as long-term migration or transient cycle in host of bovine. The recognition of animals breeding E.coli, is difficult because some adult cattle do not appear signs of illness and shedding of it and they scatter diseases in everywhere. This study is aimed at determining antimicrobial resistance of Escherichia coli o157:H7/NM isolated from raw milk. The study was conducted on bovine, camel, water buffalo, carbine and ovine in Iran. According to Clinical and Laboratory Standards Institute (CLSI), 32 isolated milk experienced for susceptibility to antimicrobial drugs by Kirby-Bauer disk diffusion test using Muller-Hinton agar. Overall 26 cases of 32 E.coli isolates were resistant to one or more antimicrobial agents. Resistance to ampicillin was the most common finding (46.6%), and after that resistance to erythromycin (31.1%), gentamycin (9.4%), nalidixic acid (12.5%), doxycyclin (904%), streptomycine (9.4%), kanamycin(904%), tetracycline (9.4%), chlorampenicol (603%), and amoxicilin( 3.1%). All E. coli isolates were sensitiveness to cefuroxime.

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