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Evaluation of Lenticular Reduced Glutathione, Ascorbic Acid and Lipid Peroxidation Levels among Cataractous Men

Author Affiliations

  • 1 Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore-641 043, Tamil Nadu, INDIA

Res. J. Recent Sci., Volume 4, Issue (4), Pages 61-65, April,2 (2015)

Abstract

Reactive oxygen species which are produced through plethora of reactions during oxidative stress in the human lens may play an important role in the formation of cataract. Lipid peroxidation may cause exacerbation of lens tissue. Glutathione and ascorbic acid are among the vital lens antioxidants that are involved in scavenging free radicals. The rationale of the present research was to assess the levels of reduced glutathione, ascorbic acid and lipid peroxidation in the eye lens among cataractous subjects. The lens samples were obtained from the ophthalmic centres in and around Coimbatore. They were categorised into apparently normal cataract men (n=15), diabetic cataract men (n=15) and hypertensive cataract men (n=15). All the parameters were analysed spectrophotometrically. The data interpretation was performed by Kruskal Wallis test. The outcome of the study showed a significant difference (p0.05) in the levels of ascorbic acid and lipid peroxidation among apparently cataract men and diabetic cataract men. However, these groups did not show significant difference albeit they showed variations in the levels of glutathione. The levels of glutathione, ascorbic acid and lipid peroxidation showed no significant difference among diabetic cataract men and hypertensive cataract men. The levels of ascorbic acid were found to have significant difference (p0.05) among apparently normal cataract men and hypertensive cataract men whereas similar trend was not observed in the levels of glutathione and lipid peroxidation.

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