5th International Young Scientist Congress (IYSC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Rhizobacteria of sugarcane: In vitro screening for their plant Growth Promoting potentials

Author Affiliations

  • 1Department of Microbiology, Shree Ramkrishna Institute of Computer Education and Applied Sciences, Surat-395001, INDIA
  • 2 Veer Narmad South Gujarat University- 395001, Surat, Gujarat, INDIA

Res. J. Recent Sci., Volume 3, Issue (IVC-2014), Pages 52-58, (2014)

Abstract

Plant growth promoting Rhizobacteria (PGPR) are group of naturally occurring soil microorganisms that aggressively colonize plant root and enhance growth of plant and yield by direct and indirect mechanism. In search for efficient PGPR strains associated to Saccharum officinarum, a total of 50 isolates were obtained from the rhizospheric soil, root, stem and leaf of sugarcane plant that were grown in agricultural field of Kamrej, Surat district, India. Among 50, eleven isolates exhibited multiple Plant growth promoting traits viz., direct mechanisms: Solubilization of Phosphate, Biological Nitrogen fixation, Zinc solubilization, Potassium solubilization, Indole-3-acetic acid (IAA) production, 1-aminocyclopropane-1- carboxylate (ACC) deaminase production, 46 isolates exhibited positively for Tri-calcium phosphate solubilization on pikovskaya agar. 39 isolates were able to fix atmospheric nitrogen, 25 isolates were able to solubilizing zinc, 24 isolates were able to solubilizing Potassium, 40 isolates were able to cleave 1-aminocyclopropane-1-carboxylate, and all isolates produced IAA in the presence of L-Tryptophan. Such multiple positive PGP traits isolates can be further explored as effective bio-inoculants to improving plant growth and yield.

References

  1. Kloepper J.W., Scrhoth M.N. and Miller T.D., Effect of rhizosphere colonization by plant growth promoting rhizobacteria on potato plant development and yield, Phytopathology,70, 1078–1082 (1980)
  2. Chen C., Bauske E.M., Musson G., Rodrigues-Kabana R. and Kloepper J.W., Biological control of Fusarium on cotton by use of endophytic bacteria, Bio Control, 5, 83-98 (1994)
  3. Zhang F., Plant growth-promoting rhizobacteria and soybean. Nodulation and fixation at suboptimal root zone temperatures, Ann Bot, 7, 453-45 (1996), 52-58 (2014)
  4. Silva VN, Silva LESF, Figueiredo MVB, Atuacao de rizobioscom rizobacterias promotora de crescimento em plantas na cultura do caupi (Vigna unguiculata L. Walp) Acta Sci Agron,28, 407-412 (2006)
  5. Kloepper J.W., Lifshitz K. and Zablotowicz R.M., Free-living bacterial inocula for enhancing crop productivity, Trends Biotechnol, 7, 39-43 (1989)
  6. Glick B.R., Karaturovíc D., Newell P., A novel procedure for rapid isolation of plant growth-promoting rhizobacteria, C and. J Microbiol,41, 533–536 (1995)
  7. Schippers B., Biological control of pathogens with rhizobacteria, Philos Trans R Soc B-Biol Sci.,318, 283-293 (1988)
  8. Cattelan A.J., Hartel P.G. and Fuhrmann J.J., Screening of plant growth promoting rhizobacteria to promote early soybean growth, J. Amer. Soil Scie. Soc., 63, 1670–1680 (1999)
  9. Glick B.R., Phytoremediation, synergistic use of plant and bacteria to cleanup the environment, Biotechnol Adv,21(3), 83-393 (2001)
  10. Dobbelaere S., Vanderleyden J. and Okon Y., Plant growth promoting effect of diazotrophs in the rhizosphere, Crit Rev Plant Sci., 22, 107-149 (2003)
  11. Zahir A.Z., Arshad M. and Frankenberger W.T., Jr., plant growth promoting rhizobacteria, application and perspectives in agriculture, Adv Agron 81, 97-168 (2004)
  12. Lucas G.J.A., Probanza A., Ramos B., Colon Flores J.J. and Gutierrez manero F.J., Effect of Plant growth promoting rhizobacteria on biological nitrogen fixation, nodulation and growth of Lupinus albus l, cv. Multolupa, Eng Life Sci., 7, 1-77 (2004a)
  13. Cakmakci R., Donmez F., Aydin A., Sahin F., Growth promotion by Plant growth promoting rhizobacteria under green house and two different soil conditions, Soil Biol Biochem,38, 1482-1487 (2006)
  14. Guo J.H., Qi H.Y., Ge H.l., Gong L.Y. and Zhang L.X., Biocontrol of tomato wilt by Plant growth promoting rhizobacteria. Biol Control 29, 66-72 (2004)
  15. Raj S.N., Deepak S.A., Basavaraju P., Shetty S.H., Reddy M.S. and Kloepper J.W., Comperative performance of formulating Plant growth promoting rhizobacteria in growth promotion and suppression of downy mildew in pearl millet, Crop Prot,22, 579-588 (2003)
  16. Saravanan V.S., Subramoniam S.R., Raj S.A., (Assessing in vitro solubilization of different zinc solubilizing bacterial (ZBS) isolates, Brazil J Microbiol,34, 121-125 (2003)
  17. Patten C.L. and Glick B.R., Role of Pseudomonas putida indole acetic acid in development of the host plant root system, Appl Environ Microbiol,68, 3795-3801(2002)
  18. Freitas De J.R., Banerjee M.R. and Germida J.J., Phosphate solubilizing rhizobacteria enhance the growth and yield but not phosphorus uptake of canola (Brassica napus L.), Biol. Fertil. Soil.,24, 358-364 (1997)
  19. Boddey R.M. and Dobereiner J., Nitrogen fixation associated with grasses and cereals: recent progress and perspectives for the future, Fert. Res.,42, 241–250 (1995)
  20. Alstrom S. and Burns R.G., Cyanide production by rhizobacteria as a possible mechanism of plant growth inhibition. Biol. Fertil. Soil., 7, 232–238 (1989)
  21. Li J., Ovakin D.H., Charles T.C. and Glick B.R., An ACC deaminase minus mutant of Entrobacter cloacae UW4 no longer promote root elongation, Curr Microbiol,41, 101-105 (2000)
  22. Penrose D.M. and Glick B.R., Methods for isolating and characterizing ACC deaminase containing plant growth promoting rhizobacteria, Physiologica Plantarum, 118, 10-15 (2003)
  23. Abdalla M.H., Phosphatases and the utilization of organic phosphorus by Rhizobium leguminosarum biovarviceae,Lett. Appl. Microbiol., 18, 294-296 (1994)
  24. Parisi B. and Vallee B.L., Metal enzyme complexes activated by zinc, J Biol Chem.,179, 803-807 (1969)
  25. Bashan Y, Inoculants of plant growth-promoting bacteria for use in agriculture, Biotechnol Adv., 16, 729-770 (1998)
  26. Zhender G.W., Yao C., Murphy J.F., Sikora E.R., Kloepper J.W., Schuster D.J. and Polston J.E., Microbes indused resistance against pathogens and herbivoras, evidence of effectiveness in agriculture: evidence of effectiveness in agriculuture, APS Press, St Paul, MN, 33 (1999)
  27. Zarrin Fatima, M. Saleemi, Muhammad Zia, T. Sultan, M. Aslam, Riaz -Ur- Rehman and M. Fayyaz Chaudhary, Antifungal activity of plant growth-promoting rhizobacteria isolates against Rhizoctonia solani in wheat, African Journal of Biotechnology,8(2), 219-225 (2009)
  28. Magnani G.S., C.M. Didonet, L.M. Cruz, C.F. Pcheth, F.O. Pedrosa and E.M. Souza, Diversity of endophytic bacteria in Brazilian Sugarcane, Genetics and Molecular research, 9(1), 250-280 (2010)
  29. Dobereiner J., Isolation and identification of root associated diaazotrophs, Plant Soil.,110, 207-212 (1989)
  30. Pikovaskya R.I., Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Microbiology, 362-370(1948)
  31. Hu X.F., Chen J. and Gu J.F., Two phosphate and potassium solubilizing bacteria isolated from Tiannu mountain, Zhejiang, China. World Journal of Microbiology and Biotechnology, 22, 983-990 (2006)
  32. Loper J.E. and M.N. Schroth, Influence of bacterial sources of indole-2-acetic acid on root elongation of sugar beet, Phytopath, 76, 386-389 (1986), 52-58 (2014)
  33. Gordon S. A. and R.P. Weber, Colorimetric estimation of indole acetic acid, Plant Physiol,26, 192–195 (1951)
  34. Arshad M. and Frankenberger Jr. W.T., Microbial production of plant growth regulators, Marcel and Dekker,New York, 307–347 (1993)
  35. Vassilev N., Vassileva M. and Nikoleva I., Simultaneous P-solubilizing and biocontrol activity of microorganisms: potentials and Future treands, Appl. Microbiol., Biotechnol.,71, 137-144 (2006)
  36. Park K.H., Lee C.Y. and Son H.J., Mechanisms of insoluble phosphate solubilization by Pseudomonas flurescens RAF15 isolated from ginseng rhizosphere and its plant growth promoting activity, Lett, Appl. Microbiol.49, 222-228 (2009)
  37. Reyes, V. A. and Z. Valduz. , Phosphate solubilizing microorganisms isolated from the rhizospheric and bulk soils of colonizer plants at an abandoned rock phosphate mine, Plant Soil287, 69-75(2006)
  38. Glick B.R., Patten C.L., Holguin G., Penrose D.M., Biochemical and genetic mechanisms used by plant growth promoting bacteria, Imperial College Press, London(1999)
  39. Mirza M.S., W. Ahmad, F. Latif, J. Haurat, R. Bally, P. Normand and K.A. Malik, Isolation, partial characterization, and the effect of plant growth-promoting bacteria (PGPB) on micro-propagated sugarcane in vitro, Plant Soil,237, 47-54 (2001)
  40. Baktharatchalu S., Shivakumar S. and Sullia S.B., Identification of multi-trait PGPR isolates and evaluation of their potential as biocontrol agents, Acta Biologica Indica.,1(1), 61-67 (2012)
  41. Mirza M.S., Rasool M. and Ashraf M.A., Nitrogen fixation and indol acetic acid production potential of bacteria isolated from rhizosphere of sugarcane ( Saccharam officinarum L.), Advan. Biol. Res., 5(6), 348-355 (2011)