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Influence of Acetone Extractive of Oroxylumindicumon Cocoon characters; Silk Filament Characters and the Electrophoretic patterns of esterase activity of silk worm Bombyxmori (L.)(Race: PM x CSR2)

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Author Affiliations

  • 1 Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Malegaon (Baramati) Dist. Pune – 413115, INDIA

Res. J. Recent Sci., Volume 3, Issue (IVC-2014), Pages 1-5,(2014)

Abstract

The present study was carried out for the purpose to know the effect of acetone extractives of stem of Oroxylumindicumon the cocoon characters; silk filament characters and esterase enzyme activity of silk worm Bombyxmori(L.) belong to the Race of PM x CSR2. The soxhletation was followed for the obtaining acetone extractive of bark of Oroxylumindicum (L). Three concentrations (5ppm; 10ppm and 20ppm) of extractive were prepared. The fifth instar larvae were utilized for the experimentation. At zero after last but the first ecdysis, the larvae fifth instar were dividedinto five groups (each with hundred individuals) (Untreated control; Acetone treated control; 5ppm extractive; 10ppm extractive and 20ppm extractive). Ten microliters of each concentration of extractives were topically applied to respective group to the individual larva at forty eight hours of age. The larvae were maintained through standard method of rearings. Ten larvae from each group were utilized for analysis of electrophoretic esterase pattern on fifth day. The silk worms were sacrificed,haemolymphand silk gland samples were isolated and analyzed by using 7.5% of native gel electrophoresis. Acetone extractives of O.indicum at 5 ppm, 10 ppm and 20 ppm concentrations recorded maximum cocoon weight (1.95,1.84,1.76 gm), shell weight (0.40, 0.35, 0.33gm), pupal weight (1.55, 1.49,1.43). All concentrations of the bark extract of O.indicum recorded higher cocoon, shell, and pupal weight than the control (untreated and acetone treated). Increase in the concentration of Oroxylum extract used for topical application was found reflected into improvement in the weight of cocoon shell followed by the shell ratio and denier scale of silk filament. Efficient use of acetone herbal extractives, likeOroxylumindicum (L) may open a new avenue in the silk yield.

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