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Influence of Acetone Extractive of Oroxylumindicumon Cocoon characters; Silk Filament Characters and the Electrophoretic patterns of esterase activity of silk worm Bombyxmori (L.)(Race: PM x CSR2)

Author Affiliations

  • 1 Shardabai Pawar Mahila Mahavidyalaya, Shardanagar, Malegaon (Baramati) Dist. Pune 413115, INDIA

Res. J. Recent Sci., Volume 3, Issue (IVC-2014), Pages 1-5,(2014)

Abstract

The present study was carried out for the purpose to know the effect of acetone extractives of stem of Oroxylumindicumon the cocoon characters; silk filament characters and esterase enzyme activity of silk worm Bombyxmori(L.) belong to the Race of PM x CSR2. The soxhletation was followed for the obtaining acetone extractive of bark of Oroxylumindicum (L). Three concentrations (5ppm; 10ppm and 20ppm) of extractive were prepared. The fifth instar larvae were utilized for the experimentation. At zero after last but the first ecdysis, the larvae fifth instar were dividedinto five groups (each with hundred individuals) (Untreated control; Acetone treated control; 5ppm extractive; 10ppm extractive and 20ppm extractive). Ten microliters of each concentration of extractives were topically applied to respective group to the individual larva at forty eight hours of age. The larvae were maintained through standard method of rearings. Ten larvae from each group were utilized for analysis of electrophoretic esterase pattern on fifth day. The silk worms were sacrificed,haemolymphand silk gland samples were isolated and analyzed by using 7.5% of native gel electrophoresis. Acetone extractives of O.indicum at 5 ppm, 10 ppm and 20 ppm concentrations recorded maximum cocoon weight (1.95,1.84,1.76 gm), shell weight (0.40, 0.35, 0.33gm), pupal weight (1.55, 1.49,1.43). All concentrations of the bark extract of O.indicum recorded higher cocoon, shell, and pupal weight than the control (untreated and acetone treated). Increase in the concentration of Oroxylum extract used for topical application was found reflected into improvement in the weight of cocoon shell followed by the shell ratio and denier scale of silk filament. Efficient use of acetone herbal extractives, likeOroxylumindicum (L) may open a new avenue in the silk yield.

References

  1. Rasadah M.A., Houghton P.J., Amala R. and Hoult J.R.S. Antimicrobial and Anti-inflammatory activity of extracts and constituents of Oroxylumindicum Vent. Phytomedica.,5, 375381 (1998)
  2. Vasanth S., Natarajan M., Sundarsan R., Rao R.B. and Kundu A.B., Ellagic acid Oroxylumindicum Vent. Indian drugs., 28(11), 507 (1991)
  3. Samatha Talari, Sampath A., Sujatha K. and Rama Swamy Nanna, Antibacterial Activity of Stem Bark Extracts of Oroxylumindicuman Endangered Ethnomedicinal Forest Tree. IOSR Journal of Pharmacy and Biological Sciences., 7(2), 2428 (2013)
  4. Warrier P.K, Nambiar V.P.K. and Raman Kutty, Oroxylumindicum, In a compendium of 500 species Indian medicinal plants, Madras, Orientlongman., IV, 186-190 (1995)
  5. Umadevi P., Venu Gopal Reddy B. and Anitha M., Synthesis and Evaluation of Diamino Substituted 1,3,4 Thiadiazole As Possible Bombyx Mori Growth Enhancer, Int. J. Pharm. Bio. Sci.,3(4), 604611 (2012)
  6. Sucheta S. Doshi, Anil N. Shendage and Vitthalrao B. Khyade, Utilization of Digixin the herbal product for treating the mulberry leaves and feeding the fifth instar larvae of silkworm, Bombyxmori (L) (Race: PM x CSR2). Standard Global Journal of Scientific Research, 1(2), 020024 (2014)
  7. Krishnaswami S., Narashimanna, Suyananrayana S.K. and Kumararaj S., Sericulture Manual 2: Silkworm Rearing, Oxford and IBH, New Delhi (1992)
  8. Thomas C.T., Szekas A., Hammock D.B., Wilson W.B. and McNamee G.M., Affinity chromatography of neuropathy target esterase, Chem. Biol. Interaction., 87,347360 (1993)
  9. Markert C.L. and Hunter R.L., The distribution of esterases in mouse tissue, J. Histochem.Cytochem.,7, 4249 (1959)
  10. He J., Studies on the inheritance of deletion type of the blood esterase isoenzymes in the silkworm, Bombyxmori L. Sericologia., 35, 1724 (1995)
  11. Stoykova T., Popov P. and Dimitrov B., Electrophoretic analysis of nonspecific haemolymphesterases during silkworm (Bombyxmori L.) ontogenesis, Sericologia., 43,153-162 (2003)
  12. Hammock .D. and Quistad G.B., In the Juvenile Hormones, ed. L. I. Gilbert (New York, Plenum Press), 374393 (1976)
  13. Akunyili D.N. Houghton P.J. and Raman A.R., Antimicrobial activities of the stem bark of Kigeliapinnata, J. Ethnopharmacol., 35, 173177 (1991)
  14. Krishnaswami S., A practical guide to mulberry silk cocoon production in tropics, published by Sriramulu, Sericulture, consultants. Bangalore, 1-10 (1994)
  15. Vitthalrao B. Khyade, Influence of Juvenoids on silkworm, Bombyxmori (L). Ph. D. Thesis, Shivaji University, Kolhapur (India) (2004)
  16. Clarke C.T., Simplified "Disc" (Polyacrylamide Gel) Electrophoresis, Ann. NY. Acad. Sci., 28(121), 428-436 (1964)
  17. Holmes R.S and Masters C.J., A comparative study of the multiplicity of mammalianesterases Biochimica et BiophysicaActa (BBA) Enzymology., 151, 147158 (1968)
  18. Reddy T.M. and Laxmipathi V., Esterases in Amolypharyngodonmola. Current Science., 57(1), 24-27 (1988)
  19. Eguchi M. and Iwamoto A., Changes in protease, esterase and phosphatases in the alimentary canal of the silkworm during meta- morphosis, Insect. Biochem., 5, 495-507 (1975)
  20. Eguchi M. and Sugimoto T., Changes in esterase zymograms in the silkworm, BombyxmoriL.,during development, J. Ins. Physiol., 11, 1145-1149 (1965)
  21. Egorova, T.L. Vasilleva, T. Sankina, Y. Nasirillaev and Y. Phillippovich, Enzymatic activity of soluble proteins in different silkworm races (B.moriL.), J. Gen. Biol., 3, 447-463 (1977)
  22. Eremina O., Studies on the effect of permethrine upon the esterase spectrum of some insects, Insect Biochem., 83-86 (1985)
  23. Raju N., Prasanna D., Bhargavi G.Y. and Venkaiah Y., Electrophoretic Patterns of Esterases in Eri silkworm Samiacynthiaricinii. IOSR Journal of Pharmacy and Biological Sciences.,7(2), 31-35 (2013)
  24. Raju N., Sampath A., Sujatha K. and Venkaiah Y., Electrophoretic Patterns of Esterases in Bombyxmori. L. Indo American Journal of Pharmaceutical Research., 3(8),6336-6340 (2013)
  25. Samatha Talari., Sampath A., Sujatha K. and Rama Swamy Nanna, Effect of stem bark extracts of Oroxylumindicuman ethno medicinal forest tree on silk production of Bombyxmori, International journal of Pharmaceutical Sciencesand Research.,(communicated) (2013)
  26. Rojas P., Mermandez L. and Pereda, Screening for antimicrobial activity of crude drug extracts and pure natural products Mexican medicinal plants, J. of Ethnopharmacology., 35, 275283 (1992)
  27. Radhika L.G., Meena C.V., Peter S., Rajesh K.S. and Rosamma M.P., Phytochemical and antimicrobial study of Oroxyumindicum,Ancient. Sci. Life., 30, 114120 (2011)