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Isolation and Characterization of L-asparginase producing isolate from Lonar Lake, Buldhana District, MS, India

Author Affiliations

  • 1Department of Biotechnology and Bioinformatics, Padmashree Dr. D. Y. Patil University, CBD Belapur , Navi Mumbai, MS, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 38-41, (2014)

Abstract

L-asparginase has emerged as one of the most important clinically used enzymes as it exhibits chemotherapeutic potential in treatment of acute lymphoblastic leukemia and lymphosarcoma. Increasing reports of Immunological responses limit the utilization of the enzyme and indicate the need of new L-asparginase with new characteristics. Besides its clinical application this enzyme is widely used in food industries to significantly reduce the formation of acrylamide a potent carcinogen in baked and fried food products. Screening of L-asparginase producing isolate from the highly alkaline and saline Lonar Lake has not been reported yet and an attempt for the same is made in this study. Out of 1592 total colonies four isolates were identified as L-asparginase producers by rapid plate assay. Further, a single isolate with the highest enzyme activity of 16.746 IU/ml was selected and identified. The organisms of the red pigmented, sticky, circular colonies having gram negative cell wall and rod shaped morphology showed positive tests for nitrate reductase, catalase and oxidase. The partial 16S rRNA sequence of the isolate showed maximum similarity with Stenotrophomonas koreensis TR6-01 strain. The alkalitolerant and halotolerant characteristic of this strain was revealed when the growth was observed in the media of pH10 and salt concentration of 4%.

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