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The Influence of Resveratrol on Swarming Differentiation and Expression of Some Virulence Factors of Proteus Vulgaris I

Author Affiliations

  • 1Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, Florida,32306, USA
  • 2Biology Department of Science College, Babylon University, IRAQ

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 396-402, (2014)


Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a stilbenoid, a type of natural phenol, and a phytoalexin with anti-inflammatory and antioxidant activities. It is produced naturally by several plants especially the roots of the Japanese Knotweed when under attack by pathogens such as bacteria or fungi. In this study we have confirmed that resveratrol has activity against Proteus vulgaris, an important pathogen infecting the urinary tract by investigating its effect on swarming and some virulence factor expression(haemolysin and urease). Swarming inhibition was limited on Luria Bertani agar with and without resveratrol and then bacteria was harvested to assay cell length and the producing of haemolysin and urease. Resveratrol significantly inhibited swarming and virulence factor expression but its effect on growth rate was not significant.


  1. Struble K.,Proteus Infections: Overview, e Medicine (2009)
  2. Burall L.S., Harro J.M., Li X., Lockatell C.V., Himps S.D., Hebe J.R., Johnson D.E. and H.L., Mobley. Proteus mirabilis genes that contribute to pathogenesis of urinary tract infection: identification of 25 signature-tagged mutants attenuated at least 100-fold, Infect Immun, 72, 2922–2938 (2004)
  3. LiawSJ, Lai HC, Ho SW, LuhK T, and WB Wang. 2000. Inhibition of virulence factor expression and swarming differentiation in Proteus mirabilis by P-nitrophenylglycerol, J MedMicrobiol, 49, 725–731 (2000)
  4. LiawSJ, Lai H.C., Ho S.W., Luh K.T. and W.B. Wang. Characterization of P-nitrophenylglycerol-resistant Proteus mirabilis super-swarming mutants, J Med Microbiol, 50, 1039–1048 (2001)
  5. Liaw S.J., Lai H.C and W.B Wang. Modulation of swarming and virulence by fatty acids through the RsbA protein in Proteus mirabilis, Infect. Immun., 72, 6836–6845 (2004)
  6. Jang J.H. and Surh Y.J., Protective effect of resveratrol on beta-amyloidal-induced oxidative PC12 cell death. Free radical Biology and Medicine., 34, 1100-1110 (2002)
  7. Zamin L.L., Dillenburg-Pilla P., Argenta-Comiran R., Horn A.P., Simao F, Nassif M, Gerhardt D, Frozza RL and Salbego C.2006. Protective effect of resveratrol against oxygen-glucose deprivation in organotypichippocampal slice cultures: Involvement of PI3-K pathway, Neurobiology of Disease, 24, 170-182 (2006)
  8. Baur J.A. and D.A. Sinclair, Therapeutic potential of resveratrol: the in vivo evidence, Nature Reviews Molecular Cell Biology, 493-506 (2006)
  9. Docherty J.J., McEwen H.A, Sweet T.J., Bailey E. and T.D. Booth, Resveratrol inhibition of Propionibacterium acnes, Journal of Antimicrobial Chemotherapy, 59, 1182-1184 (2007)
  10. Ferreira W.F. Cand Sousa J.C.F., Microbiology Volume 1.LIDEL, 1998 and 1233 (2011)
  11. Butler MS and Buss AD. Natural products--the future scaffolds for novel antibiotics?, Biochemical Pharmacology, 71, 919-929(2006)
  12. Shan B., Cai Y.Z., Brooks J.D. and Corke H., Antibacterial properties of Polygonumcuspidatum roots and their major bioactive constituents, Food Chemistry, 109, 530-537 (2008)
  13. Tombola F., Campello S., De Luca L., Ruggiero P., Del Giudice G., Papini E. and Zoratti M. Plant polyphenols inhibit Vac A, a toxin secreted by the gastric pathogen Helicobacter pylori, FEBS Letters, 543, 184-189 (2003)
  14. Mahady G.B. and Pendl S.L., Resveratrol inhibits the growth of Helicobacter pylori in vitro, American Journal of Gastroenterology, 95, 1849-1849 (2000)
  15. Tegos G, Stermitz FR, Lomovskaya O, and Lewis K. Multidrug pump inhibitors uncover remarkable activity of plant antimicrobials, Antimicrobial Agents and Chemotherapy,46, 3133-3141 (2002)
  16. McFaddin J.F., Biochemical tests for identification of medical bacteria. 1st Ed. The Williams and Wilkins, Baltimore, USA (2002)
  17. Echeverrigaray S., Michelim L., Delamare A.L., Andrade C., Costa S.O. and J.Z. acaria, The effect of Monoterpenes on swarming differentiation and Haemolysin activity in Proteus mirabilis,Molecules, 13, 3107-3116 (2008)
  18. Bulle N., Bacteria from fish and other aquatic animals: A practical identification manual. Interpretation of biochemical identification test, 121 (2004)
  19. Ray C, George, Ryan, Kenneth J, Kenneth and Ryan, Sherris Medical Microbiology: An Introduction to Infectious Diseases (4th ed.), McGraw Hill. 237 (2004)
  20. Winn W., Allen S., Janda W., Koneman E., Procop G., Schreckenberger P and G. Woods, Koneman’s color atlas and textbook of diagnostic microbiology, 6th ed. Lippincott Williams and Wilkins, Philadelphia, PA (2006)
  21. Montgomery, The analysis of variance. Experiments with a single factor: Analysis of the fixed effects model, Section, 3, 3 (2001)
  22. Allison C., Lai H.C. and C. Hughes, Co-ordinate expression of virulence genes during swarm-cell differentiation and population migration of Proteus mirabilis, MolMicrobiol, 6, 1583–1591 (1992)
  23. DekievitTR and Iglewski BH .Bacterial quorum sensing in pathogenic relationships, Infect Immun.,68, 4839–4849 (2000)
  24. ZhangL H., Quorum quenching and proactive host defense, Trends Plant Sci., 8, 238–244 (2003), 396-402 (2014)
  25. Adonizio A.L., Downum K., Bennett B.C. and Mathee K., Anti-quorum sensing activity of medicinal plants in southern Florida, J. Ethnopharmacol, 105, 427–435 (2006)
  26. Maria V., Alvarez, Maria R., Morier A. and P. Alejandra, Antiquorum Sensing and antimicrobial activity of natural agents with potential use in food, Journal of Food Safety,(2012)
  27. Fulghesu L., Giallorenzo C. and Savoia D., Evaluation of different compounds as quorum sensing inhibitors in Pseudomonas aeruginosa. ,19(4), 388-91 (2007)
  28. Vattem D., Mihalik K., Crixell S. and Mclean R., Dietary phytochemicals as quorum sensing inhibitors, Fitoterapia, 78(4), 302-310 (2007)
  29. Sturgill Gand Rather P.N., Evidence that putrescine acts as an extracellular signal required for swarming in Proteus mirabilis, Mol. Microbiol, 51, 437–446 (2004)
  30. Defoirdt T., Boon N., Bossier P. and W. Verstraete, Disruption of bacterial quorum sensing: An unexplored strategy to fight infections in aquaculture Aquaculture,240(1–4), 69–88 (2004)
  31. Sturgill G. and Rather P.N., Evidence that putrescine acts asan extracellular signal required for swarming in Proteus mirabilis, MolMicrobiol,51, 437–446 (2004)
  32. Rather P.N., Swarmer cell differentiation in Proteus mirabilis, Environ Microbiol,7, 1065–1073 (2005)