6th International Young Scientist Congress (IYSC-2020) will be Postponed to 8th and 9th May 2021 Due to COVID-19. 10th International Science Congress (ISC-2020).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Biological Control of Dengue Vector using Pseudomonas fluorescens

Author Affiliations

  • 1Department of Biotechnology, School of Biotechnology and Health sciences, Karunya University, Coimbatore 641114, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 344-351, (2014)

Abstract

In the present study Pseudomonas fluorescens from the rhizosphere soil was isolated using Kings B medium and biochemically characterised. Nutrient broth medium, Kings B medium, Kings B+ yeast extract medium and glucose peptone salt medium are used to maximize the production of exotoxin and check the anti-larvicidal activity using liquid formulation of the exoproteins which will be used for the control of dengue vector and there by eradication of the disease. The result observed that the net mortality for Kings B medium at protein concentration of 40g and 80g/ ml for 48hrs supernatant is 100% and also the 24 hrs supernatant is active against Aedes aegypti at low concentration of protein(20g/ml), while the 24hrs supernatant from glucose peptone salt medium shows 100% mortality at 80 g/ ml and Kings B yeast extract medium shows 100% mortality for 24hrs supernatant at protein concentration of 80g/ml and at 40g/ml and 80g/ml for 48 hrs supernatant. This result shows that Kings B medium is very effective for the production of exotoxin against Aedes aegypti, since it shows their larvicidal activity at low concentration of protein.

References

  1. Kovendan K., Murugan K. and Vincent S., Evaluation of larvicidal activity of Acalypha alnifolia Klein ex Willd. (Euphorbiaceae) leaf extract against the malarial vector, Anopheles stephensi, dengue vector, Aedes aegypti and Bancroftian filariasis vector, Culex quinquefasciatus (Diptera: Culicidae), Parasitology Research., 110(2), 571-581 (2012)
  2. Melo D.P.O, Scherrer R. and Eiras A.E., Dengue Fever Occurrence and ector Detection by Larval Survey, Ovitrap and MosquiTRAP: A Space-Time Clusters Analysis, PLOS neglected tropical diseases.,7(7), e42125 (2012)
  3. Malcom C.A. and Wood R.J., Location of a gene conferring resistance to knockdown by permethrin and bioresmethrin in adults of the BKPM3 strain of Aedes aegypti, Genetica (the Hague)., 59, 233-237(1982)
  4. Georghiou G.P, Wirth M, Tran H, Saume F, Knudsen A.B., Potential for organophosphate resistance in Aedes aegypti (Diptera: Culicidae) in the Caribbean Area and neighboring countries, Journal of Medical Entomology.,24, 290-294 (1987)
  5. WHO., Vector resistance to pesticides, fifteen report of the WHO expert Committee on Vector Biology and Control, Technical Report Series, No 818, WHO, Geneva, Switzerland, 62 (1992)
  6. WHO., Dengue haemorrhagic fever, diagnosis, treatment, prevention and control. 2nd edition. WHO, Geneva, Switzerland, 84 (1997)
  7. Wu Neng, Xiao Yan, Huang Fuming, Chen Dazong., Susceptibility of Aedes albopictus from China to insecticides, and mechanism of DDT resistance, Journal of the American Mosquito Control Association.,, 394-397 (1992)
  8. Rodriguez Coto M.M, Bisset Lazcano J.A., Molina de Fernandez D. and Soca A., Malathion resistance in Aedes aegypti and Culex quinquefasciatus after its use in Aedes aegypti control programs, Journal of the American Mosquito Control Association.,16, 324-330 (2000)
  9. Sadanandane C., Reddy C.M., Prabakaran G. and Balaraman K., Field evaluation of a formulation of Pseudomonas fluorescens against Culex quinquefasciatuslarvae and pupae, Acta Trop., 87, 341-3 (2003)
  10. Brammacharry U., Paily K., Chitinase like activity of metabolites of Pseudomonas fluorescens Migula on immature stages of the mosquito Culex quinquefasciatus (Diptera: Culicidae), African Journal of Microbiology Research., 6(11), 2718-2726 (2012)
  11. Prabakaran G., Hoti S. and Paily K.P., Development of cost-effective medium for the large-scale production of a mosquito pupicidal metabolite from Pseudomonas fluorescens Migula, Biological Control 48, 264266 (2009)
  12. Padmanabhan V., Prabakaran G., Paily K.P. and Balaraman K., Toxicity of a mosquitocidal metabolite of Pseudomonas fluorescens on larvae and pupae of the house fly, Musca domestica, Indian Journal of Medical Research.,121, 116-119 (2005)
  13. Meera T., Balabaskar P., Isolation and characterization of Pseudomonas fluorescens from rice fieldsInternational Journal of Food, Agriculture and VeterinarySciences., 2(1), 113-120 (2012)
  14. Cappuccino G. and Sherman N., Microbiology Laboratory manual fourth edition, first ISE reprint (1999), 344-351 (2014)
  15. Lowry O.H., Rosebrough N.J, Farr A.L. and Randall R.J., Protein measurement with the folin phenol reagent, Journal of Biological Chemistry.,193, 265-75 (1951)
  16. Reddy B.P., Reddy M.S. and Vijay Krishna Kumar K., Characterization of antifungal metabolites of Pseudomonas fluorescens and their effect on mycelial growth of Magnaporthe grisea and Rhizoctonia solani, International Journal of Pharm Tech Research., 1(4),1490-1493 (2009)
  17. Prabakaran G., Paily K.P., Padmanabhan V., Hoti S.L. and Balaraman K., Isolation of a Pseudomonas uorescens metabolite/exotoxin active against both larvae and pupae of vector mosquitoes, Pest Manag Sci.,59, 2124 (2002)
  18. Rekha V., Ahmed John S. and Shankar T., Antibacterial activity of Pseudomonas fluorescens isolated from Rhizosphere soil, International Journal of Biological Technology.,1(3),10-14 (2010)
  19. Wang Y., Fang X., An F., Wang G. and Zhang X., Improvement of antibiotic activity of Xenorhabdus bovienni by medium optimization using response surface methodology, Microbial Cell Factories.,10, 98 (2011)