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Influence of Apical Meristem and Chemotherapy on Production of Virus Free Sugarcane Plants

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Author Affiliations

  • 1Micropropagation Laboratory Sugarcane Breeding Institute, Coimbatore Tamil Nadu, INDIA
  • 2School of Biotechnology, National Institute of Technology, Calicut, Kerala, INDIA
  • 3Biotechnology, Karunya University Coimbatore Tamil Nadu, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 305-309, (2014)

Abstract

The combination of chemotherapy and the meristem culture increases the virus free production of sugarcane plants, even when the meristem is infected. In vitro culture technique has introduced a new dimension to plant multiplication for the production of well-organized, genetically unwavering clonal germplasm. The present investigation was to study the influence of meristem tip culture and the action of chemotherapy in the elimination of virus. The intention of this work is to determine the effect of various levels of chemical treatment on in vitro shoot multiplication and also assessing the elimination of virus. In this experiment three different varietiesof sugarcane were taken such as Co85004, Co91010, Co86032. These three varieties were tissue cultured using apical meristem as the explant in combination with antiviral agents of different concentrations such as 2.5mg, 5.0mg, 7.5mg, 10.0mg, 12.5mg, 15.0mg. At higher concentrations phytotoxicity was observed. About 95% virus elimination was attained with the addition of ribavirin at 10mg/l in MS medium along with meristem culture. In this experiment good response for shoot initiation was observed in variety Co 85004 compared to other varietiesstudied. The total number of days for culture establishment in Co 85004 was around 14 days. In all the three varieties the elongation was found to be more or less same in 28 – 29 days. In the case of multiplication, the number of days for shoot multiplication was only19 days in Co 86032 whereas in Co 85004 and Co 91010 it was 21 days. So, combination of antiviral chemotherapy and meristem tip culture was found to be more effective in sugarcane mosaic virus elimination.

References

  1. Rassaby L., Girard J.C., Letourmy P., Chaume J., Irey M.S., Lockhart B.E.L., Kodja H. and Rott P., Impact of Sugarcane yellow leaf virus on sugarcane yield and juice quality inReunion Island, European Journal of Plant Pathology,109(5), 459-466 (2003)
  2. Wang P.J. and Hu C., Regeneration of virus-free plants through In vitro culture. In: Advances in biochemical Engineering, (Ed.): A. Fiechter, 61-99 (1983)
  3. Springer-Verlag, Berlin N.Y. and White P.R., The Cultivation of Animal and Plant Cells, Ronald Press, New York., 59-60 (1963)
  4. Kartha K.K., Elimination of viruses in the presence of Antivirus chemicals production and inducing disease free plants, Phytopathology, 65, 219-238 (1986)
  5. Sreenivasulu P., Raju B.C. and Nayudu M.V., Carbohydrate metabolism in mosaic virus infected sugarcane, India Botanical Report, 4, 129-133 (1989)
  6. Schenck S. and Lehrer A., Factors affecting the transmission and spread of sugarcane yellow leaf virus, Plant Disease,84, 1085-1088 (2000)
  7. Kassanis B., Potato tubers freed from leaf roll virus by heat, Nature,164, 881 (1949)
  8. Nyland G. and Gohen A.C., Heat therapy of virus disease of perennial plants, Annual Review of Phytopathology,7,331-354 (1969)
  9. Mink G.I., Wample R. and Howell, Heat treatment of perennial plants to eliminate phytoplasmas, viruses and viriods while maintaining plant survival. In Plant virus disease control (Eds.) Hadidi A, Ketharpal RK and Koganezawa H, 294-300 (1998)
  10. Heinz D.J. and Mee G.W., P. Plant differentiation from callus tissues of Saccharumspecies, Crop Science,9, 346-348 (1969)
  11. Liu M.C., Sugarcane In: Handbook of plant cell culture2. (Eds.): W R Sharp, D A, Evans P V, Ammirato and Y. Yamada. Crop Science. MacMillan Publ. Co., New York, 572-605 (1983)
  12. Mori K., Production of virus free plants by means of meristem culture, Japan Agricultural Research Quarterly, 6, 1-7 (1971)
  13. Klein R.E. and Livingston C.H., Eradication of potato viruses X and S from potato shoot tip cultures with ribavirin, Phytopathology,73, 1049-1050 (1983)
  14. Parmessur Y. and saumtally A., elimination of sugarcane yellow leaf virus and sugarcane bacilliform virus by tissue cultureFood and Agricultural research council, 127–133 (2001)