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Influence of Apical Meristem and Chemotherapy on Production of Virus Free Sugarcane Plants

Author Affiliations

  • 1Micropropagation Laboratory Sugarcane Breeding Institute, Coimbatore Tamil Nadu, INDIA
  • 2School of Biotechnology, National Institute of Technology, Calicut, Kerala, INDIA
  • 3Biotechnology, Karunya University Coimbatore Tamil Nadu, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 305-309, (2014)

Abstract

The combination of chemotherapy and the meristem culture increases the virus free production of sugarcane plants, even when the meristem is infected. In vitro culture technique has introduced a new dimension to plant multiplication for the production of well-organized, genetically unwavering clonal germplasm. The present investigation was to study the influence of meristem tip culture and the action of chemotherapy in the elimination of virus. The intention of this work is to determine the effect of various levels of chemical treatment on in vitro shoot multiplication and also assessing the elimination of virus. In this experiment three different varietiesof sugarcane were taken such as Co85004, Co91010, Co86032. These three varieties were tissue cultured using apical meristem as the explant in combination with antiviral agents of different concentrations such as 2.5mg, 5.0mg, 7.5mg, 10.0mg, 12.5mg, 15.0mg. At higher concentrations phytotoxicity was observed. About 95% virus elimination was attained with the addition of ribavirin at 10mg/l in MS medium along with meristem culture. In this experiment good response for shoot initiation was observed in variety Co 85004 compared to other varietiesstudied. The total number of days for culture establishment in Co 85004 was around 14 days. In all the three varieties the elongation was found to be more or less same in 28 29 days. In the case of multiplication, the number of days for shoot multiplication was only19 days in Co 86032 whereas in Co 85004 and Co 91010 it was 21 days. So, combination of antiviral chemotherapy and meristem tip culture was found to be more effective in sugarcane mosaic virus elimination.

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