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In-vitro evaluation of Antimicrobial potency of commercially available drugs against Dentinal Caries microbes

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Author Affiliations

  • 1Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 218-223, (2014)

Abstract

Bacterial infections are common in dental practice and use of antibiotics for their treatment is also frequent. But their rampant use leads to antimicrobial resistance; a global growing issue affecting both developed and developing countries. Determining the susceptibility profile of potential pathogens is therefore necessary. Efficacy of natural phyto-chemicals isolated from plants and oral hygiene products against Streptococcus mutans; the principal dental pathogen associated with dental caries and other cariogenic bacteria isolated using the method of pure culturing has been evaluated. However, the literature lacks studies evaluating the efficacy of antibiotics against the microflora responsible for dental caries. Therefore, commercially used drugs were screened for their in-vitro antimicrobial potency against the microbial community obtained from dentinal caries lesion using Kirby-Bauer disc diffusion method. The polymicrobial growth showed susceptibility towards all the tested antibiotics except in few samples where Ampicillin/Cloxacillin, Cefixime/Clavulanic acid, Cephalexin, Cefixime and Ampicillin/ Sulbactam showed no zone of inhibition; suggesting possible resistance to such antibiotics in these patients. The comprehensive data obtained may support the polymicrobial etiology of dental caries. Such study may further allow investigation on the spatial distribution of pathogenic, antibiotic resistant bacteria among patients suffering from dental caries.

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