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Biosulphidogenesis and Bioaccumulation of sulphate by moderately Thermophilic, Facultative anaerobic Bacteria Aeromonashydrophilaisolated from hot Water spring

Author Affiliations

  • 1Department of Biotechnology, Birla College of Arts, Science and Commerce, Kalyan, Maharastra, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 203-208, (2014)

Abstract

A unique, facultative anaerobic, moderately thermophilic Sulphate reducing prokaryote (TSRP) was isolated from Vajreshwari and Ganeshpuri hot Springs of Thane, Maharashtra. The optimum temperature, pH and NaCl concentration for growth of this strain was found to 41C, 6.5, and 4.5% respectively. The strain Si showed 100% reduction ofstandardsulphatein 11hrs., with no production of sulfide. This result reflects the presence of assimilatory sulfate reduction pathway type I, which reduces sulfate to sulfite and finally to sulfide that is accumulated in the cellfor cysteine biosynthesis. The analysis of the effluent collected from colour and dye industry showed high concentration of sulphate (689ppm).These effluent wassubjected to sulphidogenesis by strain Si and there was complete reduction of sulphate in 12.30 hrs.with no production of sulphide. Phylogenetic analysis of 16s rRNA sequence placed strain Si in gamma subclass of proteobacter, showing highly similarity with other phylogenetic relatives. Thus this isolate is a member of genus Aeromonas and the type strain is hydrophila strain ZHYYZ-1. The result indicated that Aeromonashydrophila strain ZHYYZ-1 has high efficiency of sulphate reduction in much less time with no production of sulfide than previously studied anaerobic bacteria.

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