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Synergic Effects of Anticancer Drugs to Bovine Serum Albumin: A Spectroscopic Investigation

Author Affiliations

  • 1School of Chemical Sciences, Mahatma Gandhi University, Kottayam-686560, Kerala, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 157-162, (2014)


Anticancer drugs (i) 5-fluorouracil (5-FU), (ii) azacitidine (AZ) and (iii) cytarabine (CY) (pyramidine analogues) have the ability to quench bovine serum albumin (BSA).The synergic effect between the drugs and BSA were studied using fluorescence spectrophotometer and ultraviolet spectroscopic techniques under imitated physiological conditions. The results indicate that static quenching and non radiative energy transfer are the main reason of fluorescence quenching. The synergism results in both the reduction of the binding stability between drugs and BSA and an increase of the free drug concentration, which will increase the efficacy of drugs. The binding distances (r) between the drugs and BSA were obtained based on Försters theory of non-radiation energy transfer. The results indicated that the effect of synergism affected the conformation of BSA.


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