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Isolation and Characterization of Biofilm Producing Bacteria from Arabian Sea

Author Affiliations

  • 1Dept of Biochemistry and Biotechnology, S.S.V. College, Kerala, INDIA
  • 2Dept of Microbiology, Sree Narayana Guru College, K.G. Chavady, TN, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 132-136, (2014)

Abstract

Nowadays many studies have been carried out to investigate the occurrence of novel microbial bioactive compounds.Biofilms can produced by microbial species and having fascinating industrial applications. In this present study, water samples from ‘Arabian Sea', isolated organisms were screened for biofilm formation. Potent biofilm producer was identified as Halomonassp(MP) by morphological and biochemical characteristics based on Bergey’s manual of determinative bacteriology, and also by sequencing. Antibacterial activity of EPS from MP was done by disc diffusion method with some pathogenic organisms and characterized. The EPS produced in different environmental factors like incubation temperature 27C and 37C, pH 6 - 8 and in incubation time 24hr, 48hr, 72hr and 96 hr. Maximum EPS production was at pH 8, 27C and at 96 hrs of incubation time. EPS supernatant and Dry EPS produced in each parameters, quantified by total carbohydrate and total protein).

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