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Effect of sewage on Peroxidase activity, Carbohydrate, Protein and Iron content of Seedlings of Trigonella foenumgraecum (Methi)

Author Affiliations

  • 1Department of Biochemistry, Govt. Holkar Science College, Indore MP, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 95-97, (2014)


Undesirable changes in atmosphere, hydrosphere and lithospshere occuring continuouly due pollutants. Nowadays surface water bodies are getting polluted due to discharge of large amount of untreated sewage into them,a common practice of disposal. Farmers are using this sewage polluted water to irrigate their vegetable fields in city conurbations. Such irrigation practices are common to solve the problem of water shortage. The present research was done to study whether sewage water imposes raised oxidative stress condition and affects nutrients contents of crops. The study was done to observe effect of 75%, 50%, 25% diluted and 0% (undiluted) sewage of Krishnapura nallah Indore onPeroxidase Enzyme activity (marker of Stress) ,Carbohydrate, Protein and Iron content of Seedlings of Trigonella foenumgraecum (Methi). Significant reduction in peroxidase activity was observed at 50% and 75% dilution of sewage when compared with undiluted sewage i.e.0 % dilution. Reductions were also significant at all dilutions when compared with Tap water. Carbohydrate content was found to be significantly decreased at all dilutions of sewage when compared with tap water and also with undiluted sewage. Protein content was found to be significantly decreased at all dilutions of sewage when compared with tap water and also with undiluted sewage . Significant reduction in Iron content was found at 75% dilution of sewage when compared with undiluted sewage. Significant reduction in iron content was also observed with untreated sewage when compared with tap water but insignificant with 50 % and 25 % dilution of sawage. From the results of present study it is concluded that undiluted and diluted sewage were not imposing raised oxidative stress.Reduction in protein ,carbohydrate,and iron content might be due to higher amount of organic and inorganic material specially toxic heavy metals in sewage which are adversely affecting the enzymses of metabolic pathway.


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