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The effect of Nanoliposomal and PE Gylated Nanoliposomal Forms of 6-Gingerol on Breast Cancer Cells

Author Affiliations

  • 1Department of Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, IRAN
  • 2 Pilot Nanobiotechnology Department, Pasteur Institute of Iran, Tehran, IRAN
  • 3 Department of Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, IRAN

Res. J. Recent Sci., Volume 2, Issue (5), Pages 29-33, May,2 (2013)

Abstract

This study has been devoted to preparing the nanoliposomal and PEGylated nanoliposomal forms of 6-gingerol and investigating their effect on MCF-7 cell line of breast cancer. The formulations of nanoliposomal and PEGylated nanoliposomal forms of 6-gingerol were prepared through reverse-phase evaporation method and using a defined proportion of phosphatidylcholine, cholesterol, 6-gingerol, dextrano-polyethylene glycol 2000 (for PEGylated nanoliposomal form). The encapsulation rate was measured by ultracentrifugation and the drug release rate within 48-hour interval was assessed using dialysis. The MTT assay was used to determine the effect of cytotoxicity rate of formulations on the growth of breast cancer cells, and the viability and IC50 values were calculated for both formulations using the Pharm program. The rate of drug encapsulation was obtained 85% and 92% for nanoliposomal and PEGylated nanoliposomal formulations, respectively. In addition, the diameter and surface potential values were obtained 345.5 nm and -24.5 mV for nanoliposomal formulation, and 310.4 nm and -8.54 mV for PEGylated nanoliposomal form, respectively. The IC50 value for nanoliposomal and PEGylated nanoliposomal forms was obtained to be 11 and 13 g/ml, respectively. Obtained results show that the PEGylated nanoliposomal form of 6-gingerol increases its cytotoxic effect and makes the drug release slower. Therefore, the use of PEGylated nanoliposomal form of 6-gingerol helps efficiently increase its therapeutic index and decrease its side effects in treatment of breast cancer.

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