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Investigation Antibacterial Activity Extraction from two Medicinal Plants Available in Sudan

Author Affiliations

  • 1Department of Microbiology, University of Dongola, Dongola, SUDAN
  • 2 Department of Microbiology, University of El Ribat, Khartoum, SUDAN
  • 3 Department of Biochemistry, University of Alzaiem Al azhari, Khartoum north, SUDAN
  • 4 Department of Biochemistry and Molecular Biology. Faculty of Science and Technology, Al-Neelain Unversity, SUDAN

Res. J. Recent Sci., Volume 2, Issue (10), Pages 6-9, October,2 (2013)

Abstract

An aim of present study is investigated the antibacterial activity of petroleum ether and methanol extracts of two Sudanesemedicinal plants Vigna coerulea Bak. and Aloe vera were tested against three species of bacteria , Bacillus subtilis ,Escherichia coli and Staphylococcus aureus. Results were revealed the methanol extracts of two medicinal plants wereeffective on bacterial strains, while the crude plants were weakly effective. The diameter of inhibition zones ranged from 15-20 mm. A minimum inhibitory concentration (MIC) of the plants against different microorganisms was done by using amicrodillution method, it was found to be ranged from 0.156 -0.313 mg/m. In vivo-sensitivity of the plants were tested on 10,three days old by using the white rats. The rats were infected with different bacterial strains. Stained films from the lesions,cultures and histopathology confirmed the infections. The infected wounds showed dramatic response to the usage of theplants as topical treatment. Toxicity of the plants was tested in rats. The plants mixed with distilled water and given to therats as drinking water. The hematological and chemical parameters were measured before and after ingestion of the plantsby rats. Histopathology sections of the hearts, livers, lungs, kidneys and spleens of the rat become normal.

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