International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Iodometric Determination of the Ascorbic Acid (Vitamin C) content of someFruits consumed in Jimma Town Community in Ethiopia

Author Affiliations

  • 1Department of Chemistry, College of Natural Sciences, Jimma University, P.O. Box 378, Jimma, ETHIOPIA

Res.J.chem.sci., Volume 5, Issue (1), Pages 60-63, January,18 (2015)

Abstract

The objective of this study was to determine the ascorbic Acid (Vitamin C) Content of Some Fruits Consumed in Jimma TownCommunity in Ethiopia. Representative commercial fruits such as orange, lemon, papaya, mango and tomato werepurchased randomly from local market found in Jimma Town community in Ethiopia and brought to Chemistry Departmentin Jimma University and preserved in Refrigerator. The fruit samples were first washed with water; the juice from each fruitwas squeezed out, and filtered. Then, the obtained juice was centrifuged until a clear sample was obtained, which wassubsequently analyzed for Ascorbic acid content of fresh fruit juices by volumetric method. The results of present studyindicated that the concentration of ascorbic acid in each fruits found to be: Papaya (1673.018 ±136.1096 mg/100 mL),Orange (141.34 ± 22.07 mg/100 mL), Lemon (199.8133 ± 126.5819 mg/100 mL), Mango (1104.459±204.5954 mg/100 mL)and Tomato (542.002± 101.55 mg/100 mL). From the results it can be concluded that the ascorbic acid content of fruits juice(fruit pressing) were found to be in the order of Papaya> Mango > Tomato > Lemon > Orange.

References

  1. Jose L.S.B. and María Del S.S.S., Antioxidant Role of Ascorbic Acid and His Protective Effects on Chronic Diseases, http://www.intechopen.com/books/oxidativestr (2014)
  2. Nagamani k., Miracle Nutrient, IJPT, 3(2), 1140-1164 (2011)
  3. Akhilender N.K., Vitamin C in human health and disease is still a mystery? An overview, Nutr J, 2, 7 (2003)
  4. Vasanth K.G., Ajay K.K., Raghu Patel G.R. and Manjappa S., Determination of vitamin C in some fruits and vegetables in Davanagere city, (Karanataka) – India, Int j pharm life sci, 4(3), 2489 (2013)
  5. Rekha C., Poornima G., Manasa M., Abhipsa V., Pavithra Devi J., Vijay Kumar H.T. and Prashith Kekuda T. R., Ascorbic Acid, Total Phenol Content and Antioxidant Activity of Fresh Juices of Four Ripe and Unripe Citrus Fruits, Chem Sci Trans., 1(2), 303-310 (2012)
  6. Seung K.L. and Adel A.K., Preharvest and postharvest factors influencing vitamin C content of horticultural crops, Postharvest Biology and Technology, 20, 207–220 (2000)
  7. Abraha T., Subramania P.A.N., Amaha W. and Rishi P., Electrochemicaldetermination and comparison of ascorbic acid in freshly prepared and bottled fruit juices : A cyclic voltammetric study, J Chem Pharm Res, 6(5), 880-888 (2014)
  8. Maria C., Study On L-Ascorbic Acid Contents From Exotic Fruits, Cercetari Agronomice în Moldova, 1(129), 23-27 (2007)
  9. Vitamin and mineral requirements in human nutrition, Report of a joint FAO/WHO expert consultation, Bangkok, Thailand, 21–30 (1998)
  10. Gunjan K. and Mangla D.G., Analysis of Vitamin C in Commercial and Naturals substances by Iodometric Titration found in Nimar and Malwa regeion, J Sci Res Phar, 1(2), 8 (2012)
  11. Papuc C., Pop A. and Serban M., Metode Analitice in Biochimia Veterinara, Editura Printech, Bucharest, Romania, 167-169 (2001)
  12. Balan D., Pele M., Artimon M. and Luta G., Bioactive compounds in sea buckthorn fruits and in some products obtained by their processing, Rev. Cytol. Biol. Veg. Bot., 28, 364-368 (2005)
  13. Matei N., Magearu V., Birghila S. and Dobrinas S., The determination of vitamin C from sweet cherries and cherries. Revista De Chimie (Bucharest), 55, 294-296 (2004)
  14. Iwase H., Use of nucleic acids in the mobile phase for the determination of ascorbic acid in foods by highperformance liquid chromatography with electrochemical detection, J Chromatogr A, 881, 327-330 (2000)
  15. Borowski J., Szajdek A., Borowska E.J., Ciska E. and Zielinski H., Content of selected bioactive components and antioxidant properties of broccoli (Brassica oleracea L.), Eur Food Res Technol, 226, 459-465 (2008)
  16. Arya S.P., Mahajan M. and Jain P., Nonspectrophotometric methods for the determination of vitamin C, Anal Chim Acta , 417, 1-14 (2000)
  17. Vermeir S., Hertog M.L.A.T.M., Schenk A., Beullens K., Nicolai B.M. and Lammertyn J., Evaluation and optimization of high-throughput enzymatic assays for fast l-ascorbic acid quantification in fruit and vegetables, Anal Chim Acta, 618, 94-101 (2008)
  18. Aurelia M.P., Aneta P., Gheorghe P.N. and Aurel P., Determination of Ascorbic Acid Content of Some Fruit Juices and Wine by Voltammetry Performed at Pt and Carbon Paste Electrodes, Molecules, 16, 1349-1365 (2011)
  19. Hartani K.A., Micellar Catalytic Oxidation of Ascorbic Acid by Potassium Dichromate, Res. J. Chem. Sci, 4(6), 82-89, (2014)
  20. Aurelia M.P., Andrei F.D. and Slawomir K., Ascorbic Acid Determination in Commercial Fruit Juice Samples By Cyclic Voltammetry, J Autom Methods Manage Chem, 8 (2008), http://www.hindawi.com/ journals/ jammc, (2008)
  21. Analysis of commercial vitamin C tablets http://www.123 Help Me. com. of view aspid =15015 (2014)
  22. Tiruwork M. and Ghirma M., All-Solid-State Iodide Selective Electrode for Iodimetry of Iodized Salts and Vitamin C, Orient J Chem, 28(4), 1547-1555 (2012)
  23. Aydogmus Z., Etin S.M.C and Ozgur M.U., Determination of ascorbic acid in vegetables by derivative spectrophotometry, TURK J CHEM, 26(5), 697–704 (2002)
  24. Melo E.A., Lima V.L., Maciel M.I.S., Caetano A.C. and Leal F.L., Polyphenol, Ascorbic Acid and Total Carotenoid Contents in Common Fruits and Vegetables, Braz. J. Food Technol, 9, 89-94 (2006)
  25. Okiei W., Ogunlesi M., Azeez L., Obakachi V., Osunsanmi M. and Nkenchor G., The Voltammetric and Titrimetric Determination of Ascorbic Acid Levels in Tropical Fruit Samples, Int J Electrochem Sci, 4, 276- 287 (2009)
  26. Kanafe Md and Shamsul A., Analysis of vitamin C in commercial fruit juices by Iodometric Titration / Shamsul Azrin Md. Kanafe. Bachelor Degree thesis, Universiti Teknologi MARA, 1-24 (2009), SHAMSUL_AZRIN_BIN_MD._KANAFE_09_24.pdf (2014)