6th International Young Scientist Congress (IYSC-2020) will be Postponed to 8th and 9th May 2021 Due to COVID-19. 10th International Science Congress (ISC-2020).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Spectrophotometric Determination of Trace Amount of Nitrite in Water with 4-Aminophenylacetic Acid and Resorcinol

Author Affiliations

  • 1Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, NEPAL

Res.J.chem.sci., Volume 4, Issue (9), Pages 101-107, September,18 (2014)


A simple, sensitive and rapid spectrophotometric method for the determination of nitrite in different natural and wastewater samples is presented. This method is based on diazo coupling reaction where nitrite reacts with 4-aminophenylacetic acid under acidic condition to form diazonium ion which is subsequently coupled with resorcinol to form a stable orange coloured azo-dye. The λmax for the azo dye is found to be 455 nm. The optimum reaction conditions and various analytical parameters have been studied. At analytical wavelength of 455 nm the Lambert-Beer’s law obeys over the concentration range 0.1-2.4 µg/ml of nitrite. The correlation coefficient, molar absorptivity and Sandell’s sensitivity of the method were found to be 0.9986, 2.27×10-4 lit.mol-1cm-1 and 2.03×10-3 µg cm-2 respectively. The percentage recovery was found to range from 91.16% -101.4% indicating the suitability of the method for the determination of nitrite in water. The purposed study was compared with others Griess modifications and found to be suitable one. The interference of foreign ions was also examined.


  1. Hill M.J., Nitrates and Nitrites in Food and Water,st ed., Wood Head Publishing Limited, England (1996)
  2. Angela Cross, Kenneth P. Cantor, John S. Reif, Charles F. Lynch, and Mary H. Ward, Pancreatic cancer and drinking water and dietary sources of nitrate and nitrite, Am. J. Epidemiol. 159(7), 693-701 (2004)
  3. Rogers M. A. M., Vaughan T. L, Davis S., and David B. Thomas D. B, Consumption of Nitrate, Nitrite, and Nitrosodimethylamine and the Risk of Upper Aerod igestive Tract Cancer, Cancer Epidemiol. Biomarkers. Prev.,4, 29-36 (1995)
  4. Cimpeanu M., Pele M., Compeanu G. H. and Enache M., Roum. Biotech. Lett.,, 299-301 (2002)
  5. Butt S.B., Riaz M. and Iqbal M.Z., Simultaneous determination of nitrite and nitrate by normal phase ion-pair liquid chromatography, Talanta,55(4), 789-797 (2001)
  6. Siu D.C. and Henshall A., Ion chromatographic determination of nitrite and nitrate in meat products, J. Chromatogr.,804, 157-160 (1998)
  7. Barzegar L., Rezaei Behbehani G., Moosavi M. and Mehreshtiagh M., A calorimetric study on the interaction between Vitamin-B6 and lysozyme, Res. J. Recent. Sci., 1(ISC-2011),345-347 (2012)
  8. Nwajei G. E., Okwagi P., Nwajei R. I. and Obi-Iyeke G. E, Analytical assessment of trace elements in soils, tomato leaves and fruits in the vicinity of paint industry, Nigeria, Res. J. Recent. Sci., 1(4), 22-26 (2012)
  9. Chaitanya Lakshmi G., Food coloring: The natural way, Res. J. Chem. Sci., 4(2), 87-96 (2014)
  10. Khadka D. B. and Duwadi A., Spectrophotometric determination of trace amount of nitrite in water with 4-aminophenylacetic acid and phloroglucinol, Int. Res. J. Environment. Sci., 3(4), 64-69 (2014)
  11. Bassett J., Denney R. C., Jeffery G. H. and Mendham J., "Vogel's Text Book of Quantitative Analysis"th Ed. ELBS, Longman, 447 (1978)
  12. Bashir W. A. and Flamerz S., Photometric determination of nitrite, Talanta,28(9), 697-699 (1981)
  13. Amin D., Determination of nitrite on using the reaction with 4-aminobenzotrifluoride and 1-naphthol, Analyst, 111, 1335-1337 (1986)
  14. Cotton F.A. and Wilkinson G., Advanced Inorganic Chemistry, 5th ed, John Wiley and Sons Inc. (1988)
  15. Flamerz S. and Bashir W. A., Spectrophotometric determination of nitrite in waters, Analyst,106, 243-247 (1981)
  16. Veena K. and Narayana B., Spectrophotometric determination of nitrite using new coupling agents, Indian J. Chem. Technol., 16, 89-92 (2009)
  17. Pradhananga R. R., Khadka D. B. and Sherestha N. K., Spectrophotometric determination of trace amount of nitrite in water with 4-aminophenylacetic Acid and 1-naphthol, J. Nepal Chem. Soc., 11, 36-43 (1992)
  18. Cherian T. and Narayana B., A new system for the spectrophotometric determination of trace amounts of nitrite in environmental samples, J. Braz. Chem. Soc., 17(3), 577-581 (2006)
  19. Saltzman B. E.,Colorimetric microdetermination nitrogen dioxide in atmosphere, Anal. Chem., 26(12), 1949-1955 (1954)