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Spectrophotometric Determination of Trace Amount of Nitrite in Water with 4-Aminophenylacetic Acid and Resorcinol

Author Affiliations

  • 1Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, NEPAL

Res.J.chem.sci., Volume 4, Issue (9), Pages 101-107, September,18 (2014)

Abstract

A simple, sensitive and rapid spectrophotometric method for the determination of nitrite in different natural and wastewater samples is presented. This method is based on diazo coupling reaction where nitrite reacts with 4-aminophenylacetic acid under acidic condition to form diazonium ion which is subsequently coupled with resorcinol to form a stable orange coloured azo-dye. The λmax for the azo dye is found to be 455 nm. The optimum reaction conditions and various analytical parameters have been studied. At analytical wavelength of 455 nm the Lambert-Beer’s law obeys over the concentration range 0.1-2.4 µg/ml of nitrite. The correlation coefficient, molar absorptivity and Sandell’s sensitivity of the method were found to be 0.9986, 2.27×10-4 lit.mol-1cm-1 and 2.03×10-3 µg cm-2 respectively. The percentage recovery was found to range from 91.16% -101.4% indicating the suitability of the method for the determination of nitrite in water. The purposed study was compared with others Griess modifications and found to be suitable one. The interference of foreign ions was also examined.

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