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Effect of Chelating agents on Heavy Metal Extraction from Contaminated Soils

Author Affiliations

  • 1Fatima Jinnah Women University, Rawalpindi, PAKISTAN
  • 2National Agricultural Research Center, Islamabad, PAKISTAN

Res.J.chem.sci., Volume 4, Issue (9), Pages 70-87, September,18 (2014)


In the present study shacking and incubation experiment were carried out in order to evaluate the changes in heavy metal solubility in the studied soils by addition of different concentration of Ethylene dinitrilo tetra acetic acid (EDTA), Diethylene triamine penta acetic acid (DTPA), and Nitrilo tri acetic acid (NTA). The effects of EDTA, DTPA and NTA application on solubility of copper(Cu), lead(Pb), cadmium(Cd) and chromium(Cr) in soil was evaluated. In shacking experiment, maximum Cu, Pb, Cd and Cr were solubilized by DTPA extractant. It was found that with increasing chelating agent doses metals availability was increased and 5.0 mM doses of EDTA, DTPA and NTA was noticed the best optimum dose for further experiments. For shacking time significant results were achieved at 120 hours by applying EDTA and NTA where as DTPA behaved well at 24 hours. In incubation experiments more Cu and Cd was extracted by DTPA 6.65 and 6.67 ppm respectively. EDTA was proved good extracting solution for Pb which has solubilized maximum concentration of Pb (22.816 ppm). Maximum concentration of Cr (1.335 ppm) was solubilized by NTA as compared to EDTA and DTPA. For incubation experiment day 20-30 were more suitable for solubilzation of metals. Chelats has potential for the remediation of heavy metal-contaminated soils either as on-site soil washing agents or for in situ remediation. These findings could be used to develop a predictive tool for the target metals contaminants and for assessing chelation remediation efficiency based on chelates dose and contact time test results.


  1. RomoKroger C.M., Morales J.R., Dinator M.I., Llona F. and Eaton L.C., Journal ofAtmospheric Environment, 28, 705 (1994)
  2. Aragay G., Pons J., Merkoc- i, A., Chemical Reviews, 111, 3433–3458 (2011)
  3. Raskin R.D., Smith and Salt D.E., Journal of Current Opinion in Biotechnology, 221 (1997)
  4. Garbisu C., Hernandez-Allica J., Barrutia O., Alkorta I. and Becerril J.M., Phytoremediation: a technology using green plants to remove contaminants from polluted areas, Rev Environ Heal,17, 173–188 (2001)
  5. Salt D.E., Prince R.C., Pickering I.J. and Raskin I., Mechanisms of cadmium mobility and accumulation in Indian mustard, Journal of Plant Physiology, 109, 1427-1433 (1995b)
  6. Norwell W.A., Comparison of chelating agents as extractants for metals in of heavy metal contaminated soil with Indian mustard and associated potential leaching risk, Agr. Ecosyst. Environ., 102, 307–318 (1984)
  7. Felix H., Journal of Plant Nutrition and Soil Science, 160, 525 (1997)
  8. Lestan D., Luo C. and Li X., Journal of Environmental Pollution, 153, 3 (2008)
  9. Ganguly C., Matsumoto M.R., Rabideau A.J. and Van Benschoten J.E., Journal of Environmental Engineering, 124, 278 (1998)
  10. Evangelou U., Bauer M. and A. and Schaeffer A., Chemosphere, 68, 345 (2007)
  11. Kim C., Lee Y. and Ong S.K., Factors affecting EDTA extraction of lead from lead-contaminated soils, Chemosphere, 51, 845- 853 (2003)
  12. Chen H. and Cutright T., EDTA and HEDTA Effects on Cd, Cr and Ni Uptake by Helianthus annus, Chemosphere, 45(1), 21-28 (2001)
  13. Nelson D.W. and Sommers L.E., Total carbon, organic carbon and organic matter. In D.L. Sparks et al.(eds.) Methods of Soil Analysis, Part 3: Chemical Methods. Soil Science Society of America, Inc. Madison,Wisconsin, USA, 961–1010 (1996)
  14. Nelson D.W. and Sommers L.E., Total carbon, organic carbon, and organic matter: Methodsbof soil analysis, In: Chemical Methods, SSSA Book Series No. 5, pp. 961–1010b(Bartels, J. M., Ed.), Soil Science Society of America, Madison, WI (1996)
  15. Amacher M.C., Nickel, Cadmium, and Lead, Methods of Soil Analysis, Part 3, Chemical Methods. SoilScience Society of America, Book series-5, 739-768 (1996)
  16. Lai H.Y. and Chen Z.S., Effects of EDTA on Solubility of Cadmium, Zinc and Lead and Their Uptake by Rainbow Pink and Vertivers Grass, Chemosphere, 55(3), 421-430 (2004)
  17. Lombi F.E., Zhao J., Dunham S.J. and McGrath S.P., Phytoremediation of Heavy Metal Contaminated Soils: Natural Hyper Accumulation versus Chemically Enhanced Phytoextraction, Journal of Environmental Quality, 30(6), 1919-1926 (2001)
  18. Hessling J.L., Esposito M.P., Traver R.P. and Snow R.H., Results of bench scale research efforts to wash contaminated soils at battery recycling facilities, Lewis Publishers, Chelsea,Michigan, 497 (1989)
  19. Elliott H.A., Brown G.A., Shields and Lynn J.H., Restoration of lead polluted soils by EDTA extraction, 7th International Conference on Heavy Metals in the Environment, II, Geneva,64 (1989)
  20. Jean L., Bordas F. and Bollinger J.C., Journal of Environmental Pollution, 147, 729 (2007)
  21. Santos F.S., Herna΄ ndez-Allica J., Becerril J.M., Amaral-Sobrinho N., Mazur N. and Garbisu C., Chelate-induced phytoextraction of metal polluted soils with Brachiaria decumbens, Chemosphere, 65, 43–50 (2006)
  22. Lombi F.E., Zhao J., Dunham S.J. and McGrath S.P., Phytoremediation of Heavy Metal Contaminated Soils: Natural Hyper Accumulation versus Chemically Enhanced Phytoextraction, Journal of Environmental Quality,30(6), 1919-1926 (2001)