9th International Science Congress (ISC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Evaluation of Phytochemical constituents, Proximate and Fluorescence analysis of ethanolic extract and its fractions of Clerodendrumphilippinum Schauer found in Wayanad region of Kerala, India

Author Affiliations

  • 1Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala, INDIA
  • 2Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala, INDIA Department of Botany, University of Calicut, Kerala, INDIA

Res.J.chem.sci., Volume 4, Issue (9), Pages 1-6, September,18 (2014)

Abstract

Clerodendrum philippinum Schauer, a member of the family Verbenaceae, is a semi-woody shrub widely distributed in the tropics and subtropics of India. The present study was conducted forphytochemical evaluation, proximate analysis and fluorescent characteristicsof leaves of C. philippinumcollected from Western Ghats of Wayanad. The ethanolic extract and its different fractions subjected to preliminary phytochemical analysis by standard procedures. The extract revealed alkaloids, saponins, glycosides, steroids, phenolic compounds, tannins, flavonoids,fixed oils and fats.The proximateanalysis of the plant leaves revealed dry matter (94.54%), moisture (5.47%), carbohydrates(56.86%), crude fibre (11.36%), ash (8.80%), crude protein (26.25%) and crude fat (2.62%).Fluorescence analysis showed difference in characteristic colour when examined with specificchemical reagents in powdered plant leaves, crude extract and fractions of C. philippinum under visible light and UV light.

References

  1. Fredrick C. A., Onyekaba T.U., Charity C. E., Chibueze I. and Valentine C. E., Preliminary phytochemical investigations and evaluation of antimicrobial activity of n-hexane extract of the leaves of Synclisia scabrida family menispermaceae, Res. J. Pharmaceutical Sci., 2(3),1-5 (2013)
  2. Tamilarasi T. and Ananthi T., Phytochemical analysis and anti microbial activity of Mimosa pudica Linn. Res. J. Chem. Sci., 2(2), 72-74 (2012)
  3. Sandosh T. A., Peter M. P. J. and Raj J. Y., Phytochemical analysis of Stylosanthes fruticosa using UV-VIS, FTIR and GC-MS, Res. J. Chem. Sci., 3(11), 14-23 (2013)
  4. Adarsh K. T. P., Ajeesh K. T. P., Sanyo R.V.N., Juliet S., Nair S. N., Ravindran R. and Sujith S. Evaluation of phytochemical constituents and proximate contents of the ethanolic leaf extract of Tetrastigma leucostaphylum (Dennst.) Alstone (Vitaceae) found in Western Ghats of Kerala, India, Res. J. Pharmaceutical Sci., 2(10), 1-6 (2013)
  5. Hsiao J. Y. and Lin M. N., A chemotaxonomic study of essential oils from the leaves of genus Clerodendrum (Verbenaceae) native to Taiwan, Bot. Bull. Acad. Sinica.,36, 247-251 (1995)
  6. Florence A.R., Joselin J. and Jeeva, S., Intra-specific variation of bioactive principles in select members of the genus Clerodendrum L., J. Chem. Pharm. Res., 4(11),4908-4914 (2012)
  7. Neelima M., Prasad G. P., Sudarsanam G., Pratap G. P. and Jyothi B., Ethanobotanical studies in Rapur forest division of Nellore district in Andhra Pradesh, Life sci. leafl.,11, 333-345 (2011)
  8. Srivastava A., Patel S. P., Mishra R. K., Vashista R. K., Singh A. and Pushkar, A. K., Ethnomedicinal importance of the plants of Amarkantak region, Madhya Pradesh, India. Int. J. Med. Plants., 2(1), 53-59 (2012)
  9. Venkatanarasimman B., Rajeswari T. and Padmapriya B., Antibacterial Potential of Crude Leaf Extract of Clerodendrum philippinum Schauer., Intl. J. Pharm. Biol. Arch., 3(2), 307-310 (2012a)
  10. Srinivasan, Bhatt K.P., Kumar S.S., Anbu J., Rajarajan A.T., Pillai K.K., Sharma S.K. and Perianayagam J.B., Studies on anti-inflammatory activity of crude aqueous extract of Clerodendron fragrans in experimental animals, Int. J. Pharm. Sci. Lett. , 32-36 (2012)
  11. Harborne J.B., Phytochemical methods: Guide to modern techniques of plant analysis,Chapman and Hall, India 2nd edn., 653 (1991)
  12. Raaman N., Phytochemical techniques. New India Publishing Agency, NewDelhi 40 (2006)
  13. Rani N.P., Moorthi C., Senthamarai R. and Kathiresan K., A study to explore the pharmacognostic and phytochemical screening of Artemisia nilagirica leaves found in Nilgiris district of Tamil Nadu, Int. J. Pharm. Pharm. Sci.,4(4), 441-447 (2012)
  14. Pathak N.N., Kamra D.N., Agarwal N. and Jakhmola R.C.,Analytical Techniques in Animal Nutrition Research,Istedn, IBD.Co.5-23 (1996)
  15. James C. S., Analytical Chemistry of Food,Seale- Hayne Faculty of Agriculture, Food and Land use, Department of Agriculture and Food studies, University of Polymouth, UK 96-97 (1995)
  16. AOAC, Official methods of analysis of the association of the analytical chemists,Inc.Virginia, USA 17thedn., (2000)
  17. Pearson D., The Chemical Analysis of Foods, Churchill Livingstone, London 7 thedn., (1976)
  18. Chase C.R. and Pratt R., Fluorescence of powdered vegetable drugs with particular reference to development of a system of identification, J. Amer. Pharm. Assoc., 38(6),324-331 (1949)
  19. Kumar M., Mondal P., Borah S. and Mahato K.,Physicochemical evaluation, preliminary phytochemical investigation, fluorescence and TLC analysis of leaves of the plant Lasiaspinosa(Lour) Thwaites, Int. J. Pharm. Pharm. Sci., 5(2), 306-310 (2013)
  20. Prasad M.P., Sushant S. and Chikkaswamy B.K., Phytochemical analysis, Antioxidant potential antibacterial activity and molecular characterization of Clerodendrumspecies, Int. J. Mol. Boil., 3(3), 71-76 (2012)
  21. Venkatanarasimman B., Rajeswari T. and Padmapriya B., Preliminary phytochemical screening of crude leaf extract of Clerodendrum philippinum Schauer, Int. J. Institutional Pharm. Life Sci., 2(2), 133-138 (2012b)
  22. Erukainure O.L., Oke O.V., Ajiboye A.J. and Okafor O.Y., Nutritional qualities and phytochemical constituents of Clerodendrum volubile, a tropical non-conventional vegetable, Int. Food Res. J., 18(4), 1393-1399 (2011)
  23. Hussain J., Khan A.L,, Rehman N.U., Hamayun M., Shinwari Z.K., Ullah W. and In-Jung L., Assessment of herbal products and their composite medicinal plants through proximate and micronutrients analyses, J. Med. Plants Res., 3(12), 7-11 (2009)
  24. Rashida G.J.N., Venkatarathnakumar T., Aruna A.D., Gowri R., Parameshwari R., Shanthi M. and Raadhika K., Pharmacognostic and preliminary phytochemical evaluation of the leaves of dalbergia sissoo roxb, Asian J. Pharm. Clin. Res., 5(3), 115-119 (2012)