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Preliminary optimization of PHB production by Vibrio sp. MCCB 237 isolated from Marine Environment

Author Affiliations

  • 1School of Environmental Studies, Cochin University of Science and Technology, Kochi, INDIA

Res.J.chem.sci., Volume 4, Issue (5), Pages 10-13, May,18 (2014)

Abstract

Polyhydroxybutyrate (PHB) is the most widely studied polyester of microbial origin and is the representative of a group of such polyesters known as polyhydroxyalkanoates (PHA). PHAs are polymers that are synthesized by microorganisms under imbalanced growth conditions and serve as carbon and energy reserve. The material properties of PHAs are similar to various petrochemically-derived thermoplastics and elastomers that have immense use in our daily life and hence are considered as possible substitutes for synthetic, non-degradable polymers. The commercial exploitation of these polymers is still restricted by the high cost of production incurred. The success in the biodegradable plastic strategy largely depends on the isolation of potent PHB-producing bacteria and optimizing culture parameters for maximum PHB biosynthesis. In the present study the efficacy of various carbon and nitrogen sources were investigated for enhanced PHB production using Vibrio sp. MCCB 237 isolated from marine environment, which provides a meagerly exploited resource for possible biopolymer-producing novel bacteria. Quantification of PHB was done spectrophotometrically and characterized using FTIR. Among the tested carbon sources pectin followed by glycerol gave the highest yields and among the nitrogen sources, yeast extract yielded better quantity of PHB. These results are being implemented in further optimization of culture parameters.

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