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Determination of optimum period of Saccharification of water hyacinth using Trichoderma Reesei and Aspergilus Niger

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Author Affiliations

  • 1Department of Chemical Engineering, R.V. College of Engineering, Bangalore-560059, INDIA

Res.J.chem.sci., Volume 3, Issue (8), Pages 49-52, August,18 (2013)

Abstract

Alternative energy sources like bioethanol have become increasingly important in the recent past due to rapidly depleting oil reserves. Lignocellulosicfeedstocks are considered as an attractive raw material for bioethanol synthesis. Water hyacinth, an aquatic weed, tolerates variation in pH and temperature and is unaffected by toxic substances. It has high growth rates and creates problems to ecosystems and irrigation, which have called for control measures. It is found to be a suitable substrate for bioethanol production due to high cellulose and low lignin content. The two major steps involved in the conversion processes are Saccharification and Fermentation. This paper focuses on the experimental work carried out in the Saccharification stage. Pretreated Water hyacinth was added to the growth media and inoculated with Saccharification micro-organisms Trichoderma Reesei and Aspergillus Niger in different conical flasks. The optimum Saccharification period for both micro-organisms was found to be 72 hours. On testing, it was found that both organisms were capable of producing glucose, with Trichoderma Reesei (275 µg/ml) giving a better yield than Aspergillus Niger (175 µg/ml).

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