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Adsorption and Removal of Lead (Pb) by Wildtype and Lead Resistant, PbR-101 Cell Line of Chlorella sp.

Author Affiliations

  • 1Department of Chemistry, Faculty of Science, Padma Kanya Multiple Campus, Tribhuvan University, Bagbazar, Kathmandu, NEPAL

Res.J.chem.sci., Volume 3, Issue (12), Pages 16-21, December,18 (2013)


The cell line, PbR-101 isolated from Chlorella sp. by EMS (Ethylmethane sulphonate) mutagenesis was compared against the wild type (WT) Chlorella sp. for study of mechanisms confirming resistance to Pb toxicity. Growth experiments showed that both the tested algal cells, PbR-101 and WT (control) were found to be retarded with increasing Pb2+ concentrations in the liquid growth medium. It was found that the higher ID50 value of PbR-101 cell line exhibited some degree of resistance to Pb toxicity. When exposed to the liquid medium containing 50 M Pb2+, kinetic experiments revealed rapid removal and adsorption of Pb2+ in both the algal cells during the first few hours. Compared to WT, the PbR-101 cell line showed significantly higher percentage removal and adsorption of Pb2+at 15 minutes and 48 hours interval of time respectively. Extracellular Pb2+ adsorption was found significantly higher than intracellular uptake in both the tested algal cells. Total Pb2+ accumulation and distribution between the external and internal cell fractions of the PbR-101 were significantly higher to that of the WT. Thus, the PbR-101 cell line appeared more resistant to Pb toxicity and hence may be used for remediation of metal contaminated sites.


  1. Ajmal M. and Khan A.U., Effects of electroplating factory effluent on the germination and growth of hyacinth bean and mustard, Environ. Res., 38, 248-255 (1985)
  2. U.S. ATSDR, Toxicological profile for lead. (Draft for Public Comment). U.S. Department of Health and Human Services. Public Health Service. Agency for Toxic Substances and Disease Registry, Atlanta, U.S.A. (2005)
  3. Fernando N.P., Healy M.A., Aslam M., Davis S.S. and Hussein A., Lead poisoning and traditional practices: the consequences for world health. A study in Kuwait, Public Health, 95, 250-260 (1981)
  4. Kamnev A.A. and van der Lelie V., Chemical and biological parameters as tools to evaluate and improve heavy metal phytoremediation, Bioscience Reports, 20, 239-258 (2000)
  5. Salt D.E., Smith R.D. and Raskin I., Phytoremediation: Annual Review of Plant Physiology and Plant Molecular Biology49:643-668 (1998)
  6. Beker E.W., 1986. Nutritional properties of microalgae: potentials and constraints. In: Richmond A (ed)CRC Handbook of microalgal mass culture, CRC press Inc. Boca Raton, Florida, (1986)
  7. Sandau, E., Sandau, P. and Pulz O., 1996. Heavy metal sorption by microalgae, Acta Biotech., 16, 227-235 (1996)
  8. Vilchez C., Garbayo I., Lobato M.V. and Vega J.M., Microalgae-mediated chemicals production and wastes removal, Enzyme Microbial Technology 20: 562-572 (1997)
  9. Borowitzka, M.A. and Borowitzka L.J., Micro-algal Biotechnology, Cambridge University Press, Cambridge, (1988)
  10. Lopez-Suarez C.E., Castro-Romero J.M., Gonzalez-Rodrigue M.V., Gonalez-p Soto E., Perez-Iglesias J., Seco-Lago H.M. and Fernandez-Solis J.M., 2000. Study of the parameters affecting the binding of metals in solution by chlorella vulgaris, Talanta 50,1313-1318 (2000)
  11. De Filippis L.F. and Pallaghy C.K., Heavy metals: Sources and biological effects. In: Rai LC, Gaur JP and Soeder CJ (eds) Algae and water pollution. E. Schweizerbart’sche Verlagsbuchhandlung, stuttgart, (1994)
  12. Wong J.P.K., Wong Y.S. and Tam N.F.Y, Nickel biosorption by two chlorella species, C. Vulgaris (a commercial species) and C. Miniata (a local isolate), Bioresource Technology 73, 133-137 (2000)
  13. Sil A. and Chenevert J., EMS Mutagenesis, Hersckowitz Lab Protocol, Dept. of Biochemistry and Biophysics, University of California, San Francisco, (1998)
  14. Roy D., Greenlaw P.N. and Shane B.S., 1993. Adsorption of heavy metals by green algae and ground rice hull, J. Environ. Sci. Health 28, 37-50 (1993)
  15. Macfie S.M. and Welbourn P.M., The cell wall as a barrier to uptake of metal ions in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae). Arch. Environ. Contamin. Toxicol., 39, 413-419 (2000)
  16. Wong P.K. and Wong C.K., Toxicity of nickel and nickel electroplating water to Chlorella pyrenoidosa, Bull. Environ. Contamin. Toxicol., 45, 752-759 (1990)
  17. Crist R.H., Oberholder K., Schwart D., Marzoff J. and Ryder D., Interaction of metals and protons with algae, Environ. Sci. Technol., 22, 755-760 (1988)
  18. Honeyman B.D. and Santschi P.H., Metals in aquatic systems, Environ. Sci. Technol., 22, 862-871 (1988)
  19. Prasad M.N.V., Cadmium toxicity and tolerance in vascular plant, Environ. Expt. Bot., 35, 525-544 (1995)
  20. Wang H.K. and Wood J.M., Bioaccumulation of nickel by algae. Environ. Sci. Technol. 18, 106-109 (1984)
  21. Hamdy A.A., Biosorption of heavy metals by marine algae, Current Microbiology 41, 232-238 (2000)
  22. Kramer U., Cotter-Howells J.D., Charonock J.M., Baker A.J.M. and Smith J.A.C., Free histidine as metal chelator in plants that accumulate nickel, Nature 379, 653-638 (1996)
  23. Clemens S., Palmgren M.G. and Kramer U., A long way ahead: understanding and engineering plant metal accumulation, TRENDS in Plant Science , 309-315 (2002)
  24. Hamer D.H., Metallothionein, Annual Review of Biochemistry 55, 913-951 (1986)
  25. Kondo N., Imai K., Isobe M., Goto T., Murasugi A., Wada-Nakagawa C. and Hayashi Y., Cadystin A and B, major unit peptides comprising cadmium binding peptides induced in a fission yeast-separation, revision of structure and synthesis, Tetrahedron Letter 25, 3869-3872 (1984)
  26. Bajguz A., Blockage of heavy metals accumulation inChlorella vulgaris cells by 24 epibrassinolide, Plant Physiol. Biochem., 38, 797-801 (2000)
  27. Donmez C.C., Aksu Z., Ozturk A. and Kutsal T., A comparative study on heavy metal biosorption characteristics of some algae, Process Biochem., 34, 885-892 (1999)