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Adsorption and Removal of Lead (Pb) by Wildtype and Lead Resistant, PbR-101 Cell Line of Chlorella sp.

Author Affiliations

  • 1Department of Chemistry, Faculty of Science, Padma Kanya Multiple Campus, Tribhuvan University, Bagbazar, Kathmandu, NEPAL

Res.J.chem.sci., Volume 3, Issue (12), Pages 16-21, December,18 (2013)

Abstract

The cell line, PbR-101 isolated from Chlorella sp. by EMS (Ethylmethane sulphonate) mutagenesis was compared against the wild type (WT) Chlorella sp. for study of mechanisms confirming resistance to Pb toxicity. Growth experiments showed that both the tested algal cells, PbR-101 and WT (control) were found to be retarded with increasing Pb2+ concentrations in the liquid growth medium. It was found that the higher ID50 value of PbR-101 cell line exhibited some degree of resistance to Pb toxicity. When exposed to the liquid medium containing 50 M Pb2+, kinetic experiments revealed rapid removal and adsorption of Pb2+ in both the algal cells during the first few hours. Compared to WT, the PbR-101 cell line showed significantly higher percentage removal and adsorption of Pb2+at 15 minutes and 48 hours interval of time respectively. Extracellular Pb2+ adsorption was found significantly higher than intracellular uptake in both the tested algal cells. Total Pb2+ accumulation and distribution between the external and internal cell fractions of the PbR-101 were significantly higher to that of the WT. Thus, the PbR-101 cell line appeared more resistant to Pb toxicity and hence may be used for remediation of metal contaminated sites.

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