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Preparative HPLC for the Purification of major Anthocyanins from Ficus padana burm.L

Author Affiliations

  • 1Department of Chemistry, Andalas University, West Sumatra, INDONESIA

Res.J.chem.sci., Volume 3, Issue (12), Pages 60-64, December,18 (2013)

Abstract

Anthocyanin can be purified from raw extract of Ficus padana burm.L by applying preparative high performance liquid chromatography (HPLC). For every separation process, the chromatographic condition need to be optimized in order to gain the efficient purification process instantly. The current study shows that analytical scale chromatographic condition can be used as a condition for preparativehplc. Two known acylated anthocyanins are isolated from the Ficus padana burm.L by Step gradient polarity preparative HPLC. The structures are set based on spectrometric analysis. The data from each obtained anthocyanin is being compared with both mass spectral data and published data. The first compound (peak 1) was identify as a pelargonidin 3-(6”-p-coumarylglucoside)-5-(4”’-Malonylglucoside) and the second compound was identify as a pelargonidin 3-(6’’-Malonylglucoside).

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