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Assessment of the Multifaceted Immunomodulatory Potential of the Aqueous Extract of Tinospora Cordifolia

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Author Affiliations

  • 1 1Nanobiotech Lab, Dept. of Zoology, K. M. College, University of Delhi, Delhi- 110007, INDIA
  • 2 Dept. of Botany, MKR Government College, Ghaziabad, Uttar Pradesh, INDIA

Res.J.chem.sci., Volume 1, Issue (6), Pages 71-79, September,18 (2011)

Abstract

Immunomodulation relates to potentiation or suppression of the immune responses of the host, depending on the requirement of the situation. Tinospora cordifolia (T.C.), an indispensable medicinal plant, has been used for the treatment of various diseases and has been recommended for improving the immune system. In the current study, we have evaluated the cytotoxicity and immunomodulatory activity of the aqueous extract of T.C. using in vitro and ex vivo models. Results show that there was dose-dependant cytotoxicity in B16F10 mouse melanoma cells. ~90% cytotoxicity was observed 72 hours post-treatment with 100΅g/ml of T.C. extract. Interestingly, a low dose of 1΅g/ml also showed ~60% cytotoxicity. Further, the aqueous extract of T.C. exhibited boosting of phagocytic ability of macrophages, remarkable enhancement in nitric oxide production by stimulation of splenocytes and macrophages at 1mg/kg dosage of the extract. Immunomodulatory ability of a compound can be determined by its capability to influence the cytokine production, mitogenicity, stimulation and activation of immune effector cells. The extracts have been investigated for their effect on the cytokine profile (IL-6) by ELISA. Splenocytes cultured in low concentration of T.C, as low as 1.56 g/ml, produced significantly higher levels of IL-6 as compared to un-stimulated cells. The present study reveals the multifaceted immunomodulatory potential of T.C..

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