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Biodegradation of Waste gas containing mixture of BTEX by B. Sphaericus

Author Affiliations

  • 1Chemical Engineering Department, Indian Institute of Technology, Roorkee, Roorkee 247667, INDIA
  • 2Uttar Pradesh Pollution Control Board, Agra, INDIA

Res.J.chem.sci., Volume 1, Issue (5), Pages 52-60, August,18 (2011)


In the present study, a biofilter reactor was effectively applied to remove BTEX from polluted air streams. A biological study was performed by isolation in solid agar slant media and contained cell shapes was identified by using an electron microscope. It was identified for seven different isolates that this mixed culture was gram positive. These strains were cultivated on substrates with BTEX as a carbon and energy sources. From seven isolates, Bacillus sphaericus was identified for biodegration of BTEX. All isolates cultivated in a pH range from 3-11 with an optimum range of 6-8, which was applicable for the temperature range of 15-45oC giving a optimum range of 25-30oC. The batch studies were carried out at five different initial BTEX concentrations ranging from 25-500 mg L-1 . B. sphaericus was able to give 100% degradation of BTEX at 200 mg L-1 after 72 hr. but for xylene it was around 90%. Finally the results signify that the B. sphaericus degrades BTEX at a faster rate and this strain can be used proficiently in biofilter for treating highly polluted air streams.


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