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A miR302 mRNA Up-regulation Associated with Soft Tissue Sarcoma Grade

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Author Affiliations

  • 1Faculty of Medicine, University of Jambi, Letjen Soeprapto St. no. 33. Telanaipura, Jambi, Indonesia
  • 2Faculty of Medicine, University of Padjadjaran, Eykcman St. no.38. Bandung, West Java, Indonesia
  • 3Faculty of Medicine, University of Padjadjaran, Eykcman St. no.38. Bandung, West Java, Indonesia
  • 4Faculty of Medicine, University of Padjadjaran, Eykcman St. no.38. Bandung, West Java, Indonesia

Int. Res. J. Medical Sci., Volume 4, Issue (9), Pages 5-10, September,28 (2016)

Abstract

There is still a difficulty of how to diagnose and to treat the soft tissue sarcomas because its comprise of highly heterogeneous of group tumors in terms of primary site, histopathology, histological grade, and molecular signature. Histopathologic grading in adult soft tissue sarcomas is the most significant tools to predict tumor progression and metastatic risk. Tumor grading can be considered as a morphologic translation of molecular events that determine tumor aggressiveness. Therefore, molecular parameters could eventually complement or even replace histologic parameters. microRNAs (miRNAs) are small, endogenous RNAs of about 22 nucleotides that play important roles in regulation of cell proliferation, differentiation, and maintenance of “stemness”. miRNAs are negatively regulating gene expression, mainly by translational repression and play a particularly prominent role in development. In this study, we investigated profile of miR302b gene expression at level of RNA from Formalin Fixed Paraffin Embedded of low grade, intermediate grade and high grade soft tissue sarcoma using quantitative Real Time PCR (qRT-PCR). We identified 23 samples of STS cases in Jambi city, from 2007 till 2013 consist of 6 cases low grade, 9 cases intermediate grade and 8 cases high grade. miR302b gene expression was normalized to internal control by using GAPDH gene expression, and we expression using the 2-ΔΔCt method. Statistical tests were performed on groups identified to find nalyzed the relative gene iscriminating each target gene expression. There were no significant difference averages of ΔCt miR302b expression at low-, intermediate- and high- grade STS (p=0.041/ANNOVA) and also no significant with negative correlation between miR302b expression with a difference grading of STS (p>0.05, Spearman).The status of mRNA miR302bof low grade soft tissue tumor was relatively decreased expression by more than 3 fold, and relatively increased in intermediate grade and the status of high grade soft tissue sarcoma is increasing by one fold than GAPDH gene expression. It means that there is a relative change in mRNA genes expression of miR302b which is higher the histological grade more expressed the gene expression. We concluded that there was a miR302 mRNA up-regulation associated with tumor grade even no significantly but its relationship suggests that the higher grade the more expressed the miR302 gene expression.

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