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Molecular Study on Multidrug Resistant of Salmonella Enterica Isolated from patient with Enteric Fever in Najaf - Province/Iraq

Author Affiliations

  • 1College of Sciences, University of Babylon, IRAQ
  • 2 College of Medicine, University of Koffa, IRAQ
  • 3 College of Sciences, University of Koffa, IRAQ

Int. Res. J. Medical Sci., Volume 2, Issue (7), Pages 12-16, July,28 (2014)

Abstract

Minimum Inhibitory concentration of four antimicrobials used in treatment of Salmonella typhi invasive infections were determined by two different methods , the agar dilution method and HiComb kit. For Ampicillin MIC was more than 32 µg/ml. Chloramphenicol MIC ranged between (128-256) µg/ml. On other hand MIC of nalidixic acid was varied from (0.5 – 2) µg/ml. Regarding ciprofloxacin MIC range from (0.001-0.01) µg/ml. Molecular study was accomplished first by the extraction of DNA from S. typhi isolates and second by using PCR technique to amplify seven different primers to determine the presence of integrons. Genes were int, intI, for integrase gene where the first sequence gave (30%) positive while second sequence showed (20%). Sul genes were also used, the first sequence of SulI gene showed (15%) while second one gave no positive result , while sul-2 gene recorded (50%) positivity. Qac genes located on 3 end of class-I integron were also investigated , qacE did not show any positive result, while the mutant type qacE recorded high prevalence among S. typhi isolates. Finally four pairs of primers targeted the detection of Extended Spectrum Beta-Lactamases (ESBLs) genes which comprised blaTEM, blaSHV, blaOXA, and blaCTX-M. Amplification with the first three primers did not yield DNA products, while the fourth showed 50% positivity.

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