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Sub-acute effects of orally administered Ethanol extract of Root of Sarcocephalus latifolius (African peach) on Liver function markers of Wistar albino Rats

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Author Affiliations

  • 1 Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, NIGERIA
  • 2 Department of Medicine, Nnamdi Azikiwe University, Nnewi Campus, NIGERIA

Int. Res. J. Medical Sci., Volume 2, Issue (2), Pages 13-17, February,28 (2014)

Abstract

Ethanol extract of root of Sarcocephalus latifolius was administered to albino rats to determine possible effects on some liver function indices. Thirty rats, divided into six groups (A, B, C, D, E, and F) of five animals per group, were used for the study of the liver enzymes. Graded doses of the extract, 100, 200, 300, 400 and 500, mg/kg body weight, were administered orally for twenty-eight days, to test groups A – E, respectively, with group F as control (without extract). Alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) activities were determined using standard colorimetric methods according to the specifications of the manufacturer (RANDOX Laboratories Ltd, UK). A total of eighteen rats, divided into three groups of six animals each and labelled groups A, B, and C were used to study serum bilirubin. Groups A and B were orally administered 500mg/kg and 800kg/kg, body weight, for twenty-one days while group C served as control, without extract. The assays were colorimetrically done with spectrophotometer. Non-significant decreases (p >0.05) were resolved for ALP, while decrease in activities of ALT were all significant (p< 0.05), except for group E (p >0.05). There were inconsistent changes in AST activities, with all changes being non-significant (p>0.05). Decreases in both total and conjugated bilirubin were nonsignificant (p> 0.05), while nonsignificant elevations were observed for unconjugated bilirubin. The liver was apparently not affected by the root extract of Sarcocephalus latifolius to any significant extent suggesting absence of toxicity on the organ with regard to the parameters studied.

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