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Isolation and Identification of some Mycoplasma spp. from Urinary tract infection in Basrah City by Monophasic-Diphasic Culture Setup (MDCS) method and PCR

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Author Affiliations

  • 1 Department of Biology, College of Education of Pure Science, Basrah University, Basrah, IRAQ

Int. Res. J. Medical Sci., Volume 2, Issue (2), Pages 7-12, February,28 (2014)

Abstract

A total of Two hundred urine samples were collected from males and females who admitted toAl- Basrah general hospital,Al- -Sadr teaching hospital, Al –Fayhaa general hospital and Al-Mawani general hospital in center of Basrah city with signs and symptoms suggestive of UTI for the period from 1st November 2012 to April 2013, The age of patients ranged from 5-65 years. Another 50 urine samples were collected from patients attending outpatient clinics without signs and symptoms of UTI and considered as control. In an attempt to comprised isolation and identification of Mycoplasma spp and detection of bacteria other than Mycoplasma, Culture by monophasic-diphasic culture setup (MDCS) method(monophaasic-diphasic culture setup) and PCR techniques were used to detect Mycoplasma spp in genitourinary tract infections in this study. Culturing method basing on diagnostic characteristics revealed positive results of Mycoplasma spp in 87 cases(43.5%) included; Ureaplasma urealyticum in 73 cases (36.5%) while 13 cases (6.5%) were positive for M hominis and Mycoplasma fermentans in one case.(0.5%). 73 isolates of U. Urealyticum and 13 isolates of M. hominis were selected for PCR investigation. The results of this method indicated that 35/73 (47.9%) isolates of U. urealyticum were positive with PCR method versus 38/73(52%) were negative while among the 13 isolates of M. hominis, only 6/13 (46.1%) were positive with PCR technique versus 7/13 (53.8%) isolates exhibited negative results, culturing methods for diagnosis of bacteria are still the bast method. Escherichia coli was the first causative agent of infection 73(36.5%) then Staphylococcus aureus 40(20%), Klepsiella pneumoniae 25(12.5%)Pseudomonas aeruginosa 7(3.5%),Proteus2(1%).

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