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Free Radical Scavenging Activity of Metallothioneins from Peripheral Blood Lymphocytes of Gold Jewellery Karigars

Author Affiliations

  • 1 Department of Biochemistry Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher education for Women, Coimbatore-641 043, Tamilnadu, INDIA

Int. Res. J. Medical Sci., Volume 2, Issue (1), Pages 15-20, January,28 (2014)

Abstract

Metallothioneins (MT) are cysteine rich, low molecular weight metal binding proteins involved in detoxification and homeostasis of metals. Due to a variety of chemical stresses MTs are stimulated and can be used to assess human exposure to occupational agents. Human Peripheral Blood Lymphocytes (PBLs) have the ability to produce MTs against occupational exposure to silver (Ag). In the present study, PBLs were isolated from the blood of gold jewellery unit workers and MT was separated by sephadex G-75 gel filtration. Molecular mass of MT was determined by SDS-PAGE using silver staining method from the collected fractions. HPLC was performed to measure the levels of MT from the collected fraction. It is suggested that the elevated levels of MT in PBLs can be used as specific indices of silver exposure.

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