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Study of Some Bacterial Isolates Associated with Leukocytospermia in Asthenospermic Patients in Hilla City, Iraq

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Author Affiliations

  • 1Medical Microbiol.-Bacteriology, Babylon University-College of Science, IRAQ
  • 2 Medical Microbiol.-Bacteriology, Babylon University-College of Medicine,IRAQ

Int. Res. J. Medical Sci., Volume 1, Issue (3), Pages 1-11, April,28 (2013)

Abstract

One hundred asthenospermic seminal fluid specimens were collected from 100 infertile males who referred to Babylon maternity and children hospital-infertility center. It was found that 70 seminal fluid specimens had asthenospermia with leukocytospermia (First group). The rest 30 seminal fluid specimens had asthenospermia without leukocytospermia (Second group). Regarding semen cultures the results showed that 61(87.1%) of specimens of First group revealed positive bacterial culture, whereas 9(12.9%) specimens of First group showed no bacterial growth even after incubation of 48 hours. All semen specimens of Second group revealed negative bacterial culture. Gram positive bacteria constituted 44(62.9%) while gram negative bacteria constituted 26(37.1%) of isolates. Coagulase negative staphylococci (CoNS) represented by Staphylococcus epidermides and Staphylococcus saprophyticus) were the common type of bacterial isolates 25(35.7%) followed by Staphylococcus aureus 19(27.2%), Escherichia coli 12(17.1%), Enterobacter aerogenes 8(11.4%), Acinetobacter spp 4(5.7%) and Moraxella spp 2(2.9%). The virulence factors of bacterial isolates were investigated. The results showed that all S. aureus isolates, 18(72%) isolates of CoNS and 5(41.7%) of E. coli isolates and 4(50%) of E. aerogenes isolates produce hemolysin. Colonization factor antigens (CFA/) were detected in all isolates of S. aureus, CoNS, E. coli, E. aerogenes, Acinetobacter spp. and Moraxella spp. (CFA/) were expressed in 10(52.6%) isolates of S. aureus, 8(32%) isolates of CoNS, 8(66.7%) isolates of E. coli, 6(75%) isolates of E. aerogenes, 2(50%) isolates of Acinetobacter spp. and 1(50%) isolate of Moraxella spp. Lipase produced by 15(78.9%) and 7(28%) isolates of S. aureus and CoNS isolates respectively, while 9(75%) isolates of E. coli , 7(87.5%) isolates of E. aerogenes and 1(50%) isolate of Moraxella spp. produce lipase. Only 7(36.8%) isolates of S. aureus and 5(41.7%) isolates of E. coli were found to be protease producers. The effects of some antibiotics on bacterial isolates were investigated. The results showed that, the bacterial isolates were highly susceptible to imipenem, meropenem and ciprofloxacin whereas exhibited moderate resistance to amikacin, gentamycin and norfloxacin. On the other hand bacterial isolates revealed high rate of resistance to amoxicillin, ceftizoxime, ceftazidime, cefamandole, cefepime, amoxicillin–clavulanic acid and tobramycin.

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