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Preventive Effect of Shorea Robusta Bark Extract against Diethylnitrosamine -Induced Hepatocellular Carcinoma in Rats

Author Affiliations

  • 1Dept. of Biochemistry, Centre for Research and Development, PRIST University, Vallam, Tanjavur – 613403, Tamilnadu, INDIA
  • 2Department of Chemistry, PRIST University, Vallam, Tanjavur – 613 403, Tamilnadu, INDIA
  • 3Department of Biochemistry, Marudupandiyar College, Thanjavur -613 403, Tamilnadu, INDIA

Int. Res. J. Medical Sci., Volume 1, Issue (1), Pages 2-9, February,28 (2013)

Abstract

Antioxidants are one of the key players in tumorigenesis, several natural and synthetic antioxidants were shown to have anticancer effects. The aim of the present study is to divulge the preventive nature of Shorea robusta bark extract (SRBE) during diethylnitrosamine (DEN)-induced liver cancer in male Wistar albino rats. Administration of DEN to rats resulted in increased serum marker enzymes aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), and gamma glutamyl transpeptidase (GGT). The levels of lipid peroxides elevated with subsequent decrease in the tissue antioxidants like superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione peroxidase (GPx), and glutathione reductase (GR). SRBE supplementation (500mg/kg body weight) significantly attenuated these alterations, thereby showing potent anticancer effect in liver cancer. These findings suggest that SRBE prevents lipid peroxidation, hepatic cell damage, and protects the antioxidant system in DEN-induced hepatocellular carcinogenesis.

References

  1. Feo F.M., Miglio R.D., Simile M.M., Muroni M.R., Calvisi D.F., Frau M. and Pascale R.M., Hepatocellular carcinoma as a complex polygenic disease, Interpretive analysis of recent developments on genetic predisposition, Biochim Biophys Acta, 1765, 126–147 (2006)
  2. Feitelson M.A., Sun B., Tufan N.L.S., Liu J., Pan J. and Lian Z., Genetic mechanisms of hepatocarcinogenesis, Oncogene, 21, 2593–2604 (2002)
  3. Llovet J.M., Burroughs A. and Bruix J., Hepatocellular carcinoma, Lancet, 362, 1907–1917 (2003)
  4. Hala O.E.M., Metwally N.S., Soliman M.S., Ahmed K.A. and Abdel Moaty M.M., The chemopreventive effect of Ginkgo biloba and Silybum marianum extracts on hepatocarcinogenesis in rats, Cancer Cell International,11, 38 (2011)
  5. Digiovanni J., Multistage carcinogenesis in mouse skin, Pharmacol Ther, 54, 63–128 (1994)
  6. Di Stefano G., Fiume L., Bolondi L., Lanza M., Pariali M. and Chieco P., Enhanced uptake of lactosaminated human albumin by rat hepatocarcinomas: implications for an improved chemotherapy of primary liver tumours, Liver Int, 25, 854–860 (2005)
  7. Ha W.S., Kim C.K., Song S.H., Kang C.B., Study on mechanism of multistep hepatotumorigenesis in rat: development of hepatotumorigenesis, J Vet Sci, 53–58 (2001)
  8. Cheng Y.L., Change W.L., Lee S.C., Liu Y.G., Chen C.J. and Harn H.J., Acetone extract of Angelica sinensis inhibits proliferation of human cancer cells via including cell cycle arrest and apoptosis, Life Sci,75, 1579–1594 (2004)
  9. Aml F.M., Ibrahima H.M.S. and Shalaby M.A., Protective Effect of Broccoli and Red Cabbage Against Hepatocellular Carcinoma Induced by N- Nitrosodiethylamine in Rats, Journal of American Science, 6(12), 1136-45 (2010)
  10. Dhanasekaran M., Baskar A.A., Ignacimuthu S., Agastian and Duraipandiya V., Chemopreventive potential of Epoxy clerodane diterpene from Tinospora cordifolia against diethylnitrosamine-induced hepatocellular carcinoma, Invest New Drugs,27, 347–355 (2009)
  11. Mukherjee A.K., Basu S., Sarkar N., Ghosh A.C., Advances in cancer therapy with plant based natural products, Curr Med Chem,8, 1467–1486 (2001)
  12. Pezzuto J.M., Plant derived anticancer agents, Biochem Pharmacol, 53, 121–133 (1997)
  13. Kirtikar K.R., Basu B.D., Blatter E., Caius J.F. and Mahaskar K.S., Indian medicinal plants, 2nd ed. Singh B & Singh MP Publishers, 288-89 (1980)
  14. Prajapati N.D., Purohit S.S., Sharma A.K. and Kumar T., A Hand Book of Medicinnal plants, A complete source Book, 3rd Edn. Published by Agrobios (India, Jodhpur. P474, (2006)
  15. Alluri V.K., Tayi V.N.R, Sundararaju D., Vanisree M., Hsin- Sheng T. and Subbaraju G.V., Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp Artemia saina) Lethality Assay, International Journal of Applied Science and Engineering, 125-134 (2005)
  16. Reitman S. and Frankel S., A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase, Am J Clin Path, 25, 56-60 (1957)
  17. King J., The dehydrogenases or oxidoreductase-lactate dehydrogenase, In: King J (ed) Practical clinical enzymology, D Van Nostrand Company Ltd, London, 83–93 (1965)
  18. Rosalki S.B. and Rau D., Serum gamma-glutamyl transpeptidase activity in alcoholism, Clin Chim Acta, 39, 41–47 (1972)
  19. Kakkar P., Das B. and Viswanathan P.N., A modified spectrophotometric assay of SOD, Ind J Biochem Biophy, 21, 130-132 (1984)
  20. Beers R. and Sizer I., A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase, J. Biol Chem., 195, 133 (1952)
  21. Rotruck J.T., Pope A.L., Ganther H.E., Swanson A.B., Hafeman D.G. and Hoekstra W.G., Selenium: biochemical roles as component of glutathione peroxidase, Sci 1973; 179: 588-590. Baker H, Frank O, De Angeles B, Feinglod S. Plasma tocopherol in man at various times after ingesting free or acetylated tocopherol. Nutr Repor Inter, 21, 531 (1980)
  22. Stall G.E.J., Visser J. and Veeger C., Purification and properties of glutathione reductaase in human erythrocytes, Biochem. Biophys. Acta., 185, 39-48 (1969)
  23. Moron M.S., DsePierre J.W. and Manerwik K.B., Levels of glutathione, glutathione reductase and glutathione-s-transferase activities in rat lung and liver, Biochim Biophy Acta, 582, 67-68 (1979)
  24. Bulle F., Mavier P., Zafrani E.S., Preaux A.M., Lescs M.C., Siegrist S., Dhumeaux D. and Guellaen G., Mechanism of gamma-glutamyl transpeptidase release in serum during intrahepatic and extrahepatic cholestasis in the rat: a histochemical, biochemical and molecular approach, Hepatology, 11, 545–550 (1990)
  25. Lippert M., Papadopoulos N. and Javadpour N.R., Role of lactate dehydrogenase isoenzymes in testicular cancer, Urology, 18, 50–53 (1981)
  26. Ramakrishnan G., Augustine T.A., Jagan S., Vinodkumar R. and Devaki T., Effect of silymarin on N-Nitrosodiethylamine induced hepatocarcinogenesis in rats, Exp Oncol, 29, 39–44 (2007)
  27. Whittby L.G., Perey-Robb I.W. and Smith A.T., Enzymes tests in diagnosis, Lecture notes in clinical chemistry, 3rd edn. Black Well Scientific Publications, London, 138–169 (1984)
  28. Sun Y., Free radicals, antioxidant enzymes, and carcinogenesis, Free Radic Biol Med., 583–599 (1990)
  29. Halliwell B., Free radicals, antioxidant and human disease: curiosity, cause or consequence? Lancet, 344, 721–724 (1994)
  30. Halliwell B., Gutteridge J.M.C., Protection against oxidants in biological systems: the superoxide theory of oxygen toxicity, In: Cheeseman KH (ed) Free radicals in biology and medicine, Clarendon Press, Oxford, 144–147 (1989)
  31. Gey K.F., Prospects for the prevention of free radical disease, regarding cancer and cardiovascular disease, Br Med Bull49, 679–699 (1993)
  32. Banakar M.C., Paramasivan S.K., Chattopadhyay M.B., Datta S., Chakraborty P., Chatterjee M., Kannan K., Thyagarajan E. a1, 25-dihydroxyvitamin D3 prevents DNA damage and restores antioxidant enzymes in rat hepatocarcinogenesis induced by diethylnitrosamine and promoted by Phenobarbital, World J Gasteroenterol,10, 1268–1275 (2004)
  33. Hietanen E., Ahotupa M. and Bartsch H., Lipid peroxidation and chemically induced cancer in rats fed lipid rich diet, In: Lapis K (ed) In carcinogensis and tumor progression, Akademiaikiado Press, Budapest, 9–16 (1987)
  34. Klaunig J.E. and Kamendulis L.M., The role of oxidative stress in carcinogenesis, Annu Rev Pharmacol Toxicol, 44, 239–267 (2004)
  35. Hirose M., Imaida K. and Tamano S., Cancer chemoprevention by antioxidants, In: Ho CT (ed) Food phytochemicals: teas, spices and herbs, American Chemical Society Press, Washington, 122–132 (1994)
  36. Sen C.K., Oxygen toxicity and antioxidants: state of the art, Int J Physiol Pharmacol, 39, 177–196 (1995)
  37. Gutteridge J.M., Antioxidants, nutritional supplement and life threatening diseases, Br J Biomed Sci., 51, 288–295 (1994)
  38. Feng Q., Kumagai T., Torii Y., Nakamura Y., Osawa T. and Uchida K., Anticarcinogenic antioxidants as inhibitors against intracellular oxidative stress, Free Radic Res., 35, 779–788 (2001)
  39. Oberley L.W. and Oberley T.D., Free radicals, cancer and aging. In: Johnson J (ed) Free radicals, aging and degenerative diseases, Alan R Liss Inc, New York, 325–371 (1986)
  40. Mccord J.M. and Fridovich I., The utility of superoxide dismutase in studying free radical reactions, I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen, J Biol Chem, 244, 6056–6063 (1969)
  41. Chasseaud L.F., The role of glutathione and glutathione-stransferase in the metabolism of chemical carcinogens and other electrophilic agents, Adv Cancer Res, 29, 175–274 (1979)
  42. Sumathi R., Baskaran G. and Varalakshmi P., Effect of DL-lipoic acid on tissue redox state in acute cadmium-challenged tissues, J Nutr Biochem, 7, 85–92 (1996)