In vitro anti-arthritic activity of vitex negundo and punica granatum
- 1Department of Biochemistry, Shri. Shivaji College of Artís, Commerce and Science, Akola, Maharashtra, India
- 2Department of Biochemistry, Shri. Shivaji College of Artís, Commerce and Science, Akola, Maharashtra, India
- 3Department of Biochemistry, Shri. Shivaji College of Artís, Commerce and Science, Akola, Maharashtra, India
- 4Department of Biochemistry, Shri. Shivaji College of Artís, Commerce and Science, Akola, Maharashtra, India
- 5Department of Biochemistry, Shri. Shivaji College of Artís, Commerce and Science, Akola, Maharashtra, India
Res. J. of Pharmaceutical Sci., Volume 6, Issue (2), Pages 5-7, February,30 (2017)
The aim of present study is to evaluate the in vitro anti arthritic activity of aqueous and ethanolic extract of Vitex negundo and Punica granatum by Bovine serum albumin and egg albumin denaturation method. The activity of aqueous and ethanolic extract of Vitex negundo and Punica granatum was compared with standard Anti -arthritic drug Diclofenac Sodium. Results revealed that the ethanolic extract of Punica granatum at a different concentration possessed significant anti- arthritis activity as compare to std. drug. The current finding exhibited a concentration dependent inhibition of protein (egg albumin and BSA) denaturation by aqueous and Ethanolic extract of Vitex negundo and Punica granatum. The effect of Diclofenac Sodium was found to be approximate when compared with the Punica granatum at (800Ķg/ml). In denaturation method egg albumin, at concentration of 100, 200, 400 and 800Ķg/ml, ethanolic extraction of Vitex negundo showed 40.11%, 58.05%, 63.43% and 87.52% respectively. Whereas, ethanolic extraction of Punica granatum showed 50.28%, 101.71%, 187.75%, 261.08%. In Bovine Serum albumin denaturation method at concentration of 100, 200, 400 and 800Ķg/ml, ethanolic extraction of Vitex negundo showed 20.03%, 42.72%, 59.06%, 72.08% respectively. Whereas, Ethanolic extraction of Punica granatum showed 28.54%, 42.88%, 81.39%, 99.18% of inhibition. From the present finding it can be concluded that Punica granatum possessed maximum anti-arthritic effect against denaturation of protein invitro. The effect was possibly due to flavonoids, Alkaloids, terpenoids content of Punica granatum.
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