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Evaluation of phytochemical constituents and proximate contents of the ethanolic leaf extract of Tetrastigmaleucostaphylum (Dennst.) Alstone (Vitaceae) found in Western Ghats of Kerala, India

Author Affiliations

  • 1Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode-673 576, Wayanad, Kerala, INDIA
  • 2 Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode-673 576, Wayanad, Kerala, INDIA

Res. J. of Pharmaceutical Sci., Volume 2, Issue (10), Pages 1-6, November,30 (2013)


The presentstudy aims at determining the phytochemicaland proximate contents of the leaves of Tetrastigmaleucostaphylum (Dennst) Alstone (family: Vitaceae). The leaf of the plant is being commonly used by tribals of Wayanad for its various medicinal properties. The ethanolic leaf extract was analysed for various phytochemical constituents using standard procedures. The analysis of ash, crude fibre, crude protein, carbohydrate, crude fat, dry matter and moisture content of the leaves were carried out using standard proximate analysis techniques. The leaves were found to contain phytochemicals such as alkaloids,phytosteroids, saponin, diterpenes, cardiac glycosides, carbohydrate, fixed oils and fats.The result of the proximate contents showed that the dry matter has maxima of 93.15%, the moisture content 6.85%, carbohydrate 58.97% while crude fibre has a percentage of 12.00%, ash 13.78%, crude protein 17.50% and crude fat 2.90%.


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