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Antitumor and Immunomodulatory Activity of Phallusia nigra Savigny, 1816 Against Ehrlich Ascites Carcinoma

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Author Affiliations

  • 1 Department of Zoology, A.P.C. Mahalaxmi College for Women, Tuticorin-628002, Tamil Nadu, INDIA

Res. J. of Pharmaceutical Sci., Volume 1, Issue (2), Pages 7-12, October,30 (2012)

Abstract

Antitumor and Immunomodulatory activity of the ethanol extract of the simple ascidian Phallusia nigra was assessed against Ehrlich Ascites Carcinoma (EAC) cells. 100% toxicity was observed at a concentration of 0.40 mg/ml. The extract at 50, 100, 150 mg/kg body weight and standard drug Vincristin at 0.08 mg/kg was administered to different groups. The results showed significant reduction in weight of body, thymus, lungs and an increase in the weight of spleen, liver and kidney. There was a decrease in tumor volume, packed cell volume, viable cell count and increase in nonviable cell count and median survival time there by an increase in lifespan. Hematological parameters were restored to normal. The bone marrow cellularity and β esterase positive cells enhanced significantly. The maximum number of plaque forming cells was observed in group IV on the 6th day and a significant antibody titer (211.82) on the 15th day of treatment. Reduction in serum Gamma Glutamyl Transpeptidase (GGT), Glutathione (GSH) and nitric oxide (NO) was noticed. The above results indicate the presence of bioactive compounds in ascidians.

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